Cell 170, 1120–1133 (2017). CAS PubMed Central Google Scholar Lee, E. H. J. et al. Antigen-dependent IL-12 signaling in CAR T cells promotes regional to systemic disease targeting. Nat. Commun. 14, 4737 (2023). ADS CAS PubMed Central Google Scholar Gao, S., Hsu, T. W. &...
Chromium Next GEM Single Cell 5’ Kit v2 (10× Genomics) (PN-1000263) was used to perform single-cell separation and library construction according to the manufacturer’s instructions. Briefly, the cell suspension, barcoded gel beads and partitioning oil were loaded onto the 10x Genomics ...
Analysis of cytokine concentrations were done using Milliplex MAP Mouse CD8+ T Cell Magnetic Bead Panel Premix 15 Plex (MCD8MAG48K-PX15) according to the manufacturer’s instructions. A MAGPIX system (Luminex) was used for data acquisition. DNA extraction and 16S rRNA gene sequencing of the ...
(Sense: 5′-UUCUCCGAACGUGUCACGUTT-3′; Antisense: 5′-ACGUGACACGUUCGGAGAATT-3′) (Genepharm, Shanghai) for 72 h using lipofectamine RNAiMAX transfection reagent (Thermo Fisher) according to the manufacturer’s instructions (https://www.thermofisher.cn/order/catalog/product/13778075?SID=srch...
Using cDNA as a template and β-actin as an internal reference, the cDNA template obtained by reverse transcription was added to the detection system according to the RT-PCR reagent operating instructions. The ABI7500 fluorescence RT-qPCR system (Applied Biosystems, Germany) was used to detect ...
Immune checkpoint inhibitors (CPIs) are a relatively newly licenced cancer treatment, which make a once previously untreatable disease now amenable to a potential cure. Combination regimens of anti-CTLA4 and anti-PD-1 show enhanced efficacy but are prone
RNA was extracted using NucleoSpin® RNA II kit (Macherey-Nagel, Hoerdt, France) according to the manufacturer’s instructions. Reverse transcription was performed using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). Real-time polymerase chain reaction (PCR) was performed using Brill...
ELISA kits (Biolegend, Cat# 439407, Cat# 431307, Cat# 430907) were used for examining the concentrations of cytokines in cell supernatants or serum of mice according to the manufacturer’s instructions. Flow cytometry assay Cell preparations and surface staining were performed as the method details...
ST2hi memory-type Th2 cells are identified as a pathogenic subpopulation in eosinophilic airway inflammation. These ST2hi pathogenic Th2 cells produce large amount of IL-5 upon T cell receptor stimulation, but not in response to IL-33 treatment. By contr
20Citations 10Altmetric Metrics Abstract Acute or chronic metabolic complications such as diabetic ketoacidosis are often associated with extracellular acidification and pancreatic β-cell dysfunction. However, the mechanisms by which human β-cells sense and respond to acidic pH remain elusive. In this ...