Chromium Next GEM Single Cell 5’ Kit v2 (10× Genomics) (PN-1000263) was used to perform single-cell separation and library construction according to the manufacturer’s instructions. Briefly, the cell suspension, barcoded gel beads and partitioning oil were loaded onto the 10x Genomics ...
42 Briefly, feces were collected from mice and fungal genomic DNA was isolated from the feces and from colons with use of the QIAamp DNA Stool Mini Kit (Qiagen) according to the manufacturer’s instructions. Fungal 18S rDNA was subsequently evaluated by using qPCR analysis. Fungal burden was ...
Using cDNA as a template and β-actin as an internal reference, the cDNA template obtained by reverse transcription was added to the detection system according to the RT-PCR reagent operating instructions. The ABI7500 fluorescence RT-qPCR system (Applied Biosystems, Germany) was used to detect ...
Induction ofDusp10suppresses IL-5 production in ILC2.aSchematic representation of wild-typeDusp10(WT) and C409S mutant.b–gFreshly prepared ILC2s purified form the spleen of IL-33-injected BALB/c mice were infected with a Mock (empty vector),Dusp10, or C409S mutant-IRES-hNGFR-containing...
Membranes. 2023; 13(1):26. https://doi.org/10.3390/membranes13010026 Chicago/Turabian Style Vroulias, Dionysios, Eirini Staurianou, Theophilos Ioannides, and Valadoula Deimede. 2023. "Poly(ethylene oxide)-Based Copolymer-IL Composite Membranes for CO2 Separation" Membranes 13, no. 1: 26....