Total RNA was isolated from highly pure, sorted human ILC subsets using the TRIZOL reagent according to the manufacturer’s instructions (Invitrogen). Final preparation of RNA was considered DNA- and protein-free if the ratio of readings at 260/280 nm was ≥1.7. Isolated mRNA was reverse-tr...
ELISA was performed according to the manufacturer’s instructions. The absorption was measured at 450 nm. The protein concentration was determined by comparing the relative absorbance of the samples with the standards. Each result was repeated three times. Quantitative real-time PCR (RT-qPCR) RT...
14. There are important concerns that immunosuppression might impede the anti-cancer responses of CPI therapy. Two independent studies in different tumour settings, identified impaired survival in steroid exposed patients15,16. Treatment outcomes with biological therapies, such as anti...
14. There are important concerns that immunosuppression might impede the anti-cancer responses of CPI therapy. Two independent studies in different tumour settings, identified impaired survival in steroid exposed patients15,16. Treatment outcomes with biological therapies, such as anti...