To understand how immune checkpoint perturbation impacts the colonic immune system, we administered anti-CTLA4/anti-PD-1 combination therapy to wild-type (WT) Balb/C mice. Combination checkpoint inhibition triggered systemic immune activation with induction of splenomegaly, even after 1 week of treatmen...
ELISA was performed according to the manufacturer’s instructions. The absorption was measured at 450 nm. The protein concentration was determined by comparing the relative absorbance of the samples with the standards. Each result was repeated three times. Quantitative real-time PCR (RT-qPCR) RT...
To understand how immune checkpoint perturbation impacts the colonic immune system, we administered anti-CTLA4/anti-PD-1 combination therapy to wild-type (WT) Balb/C mice. Combination checkpoint inhibition triggered systemic immune activation with induction of splenomegaly, even after 1 week of treatmen...
Immunohistochemical staining was performed using the fully automated immunostainer Benchmark XT (Roche, Basel, Switzerland). Staining protocol included deparaffinization and counterstain with hematoxylin and blueing reagent according to the manufacturers´ instructions. The IL-6 antibody required pretreatment b...
ST2hi memory-type Th2 cells are identified as a pathogenic subpopulation in eosinophilic airway inflammation. These ST2hi pathogenic Th2 cells produce large amount of IL-5 upon T cell receptor stimulation, but not in response to IL-33 treatment. By contr