The present disclosure relates to a symmetric bispecific antibody of the class IgG4 comprising two heavy chains which each comprise a variable domain, CH1 domain and a hinge region, wherein in each heavy chain the inter-chain cysteine at position 127, numbered according to the Kabat numbering ...
[0015] a.ChI结构域中根据Kabat编号系统编号的位置127上的链间半胱氨酸被另一个氨 基酸置换;和[0016] b.位于上铰链区(upper hinge region)中的一个或多个氨基酸被半胱氨酸置换。[0017] 与轻链中的半胱氨酸形成链间二硫键的ChI结构域中的半胱氨酸为根据Kabat编 号系统编号的位置127上的半胱氨酸,且示于图...
pFUSE-hIgG4e1-Fc1is a cloning plasmid for the generation of aFc-fusion proteinexpressing the Fc region (CH2 and CH3 domains) of thehuman IgG4 heavy chain mutatedS228Pand thehinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each par...
SEQUENCE SYMMETRIC MODIFIED IGG4 BISPECIFIC ANTIBO 专利名称：SEQUENCE SYMMETRIC MODIFIED IGG4 BISPECIFIC ANTIBODIES 发明人：HUMPHREYS, David, Paul,PETERS, Shirley, Jane 申请号：EP2013/053614 申请日：20130222 公开号：WO2013/124450A1 公开日：20130829 专利内容由知识产权出版社提供 专利附图：摘要：The ...
The constant region of Anti-hCD20-hIgG4 (S228P) contains the S228P mutation which reduces Fab-arm exchange by stabilizing the disulfides in the core-hinge of the IgG4 molecules [1]. Anti-hCD20-hIgG4 (S228P) was generated by recombinant DNA technology. It has been produced in Chinese ...
Antibody determinants that influence the affinity for Fcγ receptors include sequence variation in the Cγ2 domain and hinge region, and the composition of the oligosaccharide moiety attached to the Cγ2 domain (Canfield and Morrison, 1991, Shields et al., 2002). The Cγ2 domain FG loop (...
The sequence of anti-LAG3 was used to construct the monovalent IgG. Mutations in the CH1 and CL domains of H chain (pcDNA3.4-HC.knob) and L chain (pcDNA3.4-LC) transfer vectors were introduced by PCR. Another heavy chain Fc that only has the hinge, CH2 and CH3 was constructed as ...
Abbreviations: Fc, fragment crystallization region; G1, IgG1; G4, IgG4; and G1-CH3, IgG1 CH3 domain Full size image REGN-H098P mAbs Engineered with Reduced Pre-existing Reactivity Different versions of REGN-H098P were subsequently engineered that contained wild-type IgG1 sequences in the ...
34 It is considered that the hinge region acts as a spacer, effecting segmental flexibility and allowing the Fab arms to move in relation to the Fc.35 A study showed that changes in the amino acid sequences could affect the rigidity of the hinge, which in turn affected the effector ...
Therapeutic antibodies may rely on their constant region (Fc domain) ability to induce target cell killing via immune-mediated effector functions, such as ADCC and CDC to achieve efficacy. Human IgG1 and IgG3 antibodies have Fc domain sequences that can mediate potent effector functions, while hu...