此外,IGF2BP1-KO显著增加了AGO2与MYCN mRNA的结合。在神经母细胞瘤细胞模型中,IGF2BP1促进MYCN表达,主要依赖于3'UTR、miRNA和BTYNB,独立于m6A的修饰。图2 图2 IGF2BP1促进MYCN的表达依赖于3'UTR、miRNA和BTYNB 3 IGF2BP1是神经母细胞...
1、本发明的目的在于提出m6a阅读蛋白igf2bp1在废用性骨质疏松症诊断和治疗药物中的应用,首次发现了m6a阅读蛋白igf2bp1在废用性骨质疏松症中的变化,探究了其对成骨细胞增殖功能以及骨形成的影响,提示其可能作为废用性骨质疏松症潜在的生物标志物,并可作为新的靶点对抗废用性骨质疏松症,具有良好的转化医学前景。 2、...
HCC肿瘤组织(T)相对于正常组织(N)中m6A修饰因子的差异表达;FC=倍数变化。 RIP-qPCR实验显示HUH1细胞中与IGF2BP1结合的EPHA3和VIM-AS1 RNA。 IGF2BP1沉默对EPHA3 mRNA表达的影响。 对包括m6A位点在内的EPHA3 3′UTR野生型(WT)和突变型(MUT)构建体的MeRIP-qPCR分析。 在IGF2BP1沉默后使用WT和MUT构建体进行...
本文主要是发现m6A读取蛋白IGFBP1的敲低可以诱导肿瘤细胞凋亡,以及发现小分子CuB可以与该蛋白共价结合并激活肿瘤免疫微环境。 mRNA的m6A修饰在真核细胞的多种生物学进程中起到非常重要的作用,而IGF2BPs蛋白可以识别这种修饰并通过招募辅因子来...
2022 Elsevier Inc.The N6-methyladenosine (m6A) is involved in the regulation of cell proliferation and metastasis formation in multiple cancers. However, the biological significance of RNA m6A reader IGF2BP1 and the modification of IGF2BP1 itself have not been fully investigated. Here, we analyzed...
IGF2BP1 bound to HMGB1 mRNA and enhanced its stability by m6A modification. Conversely, when IGF2BP1 was knocked down and HMGB1 was overexpressed, the results were opposite to those observed previously. Conclusion IGF2BP1 promotes endometriosis progression by enhancing m6A modification stability of ...
摘要: 研究目的:肿瘤干细胞(CSC)是导致肿瘤复发与转移的重要因素.m6A读码器IGF2BP1与口腔鳞癌发生发展密切相关.但IGF2BP1对口腔鳞癌干细胞的调控作用尚不清楚.本项目拟探究IGF2BP1调控口腔鳞癌干细胞的分子机制,为靶向口腔鳞癌干细胞提供新的靶点.关键词: 口腔鳞状细胞癌、肿瘤干细胞、IGF2BP1、FOXK1、m6A ...
m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3 Authors - Ting Shan KD Validated View Article human,mouse WB Adv Sci (Weinh) SREBP1c-Mediated Transcriptional Repression of YME1L1 Contributes to Acute Kidney Injury by Inducing Mitochondrial Dy...
IGF2BP1 stabilized SIK2 mRNA through m6A modification to promote NSCLC progression, potentially offering new diagnostic and therapeutic insights for NSCLC. Introduction Non-small cell lung cancer (NSCLC) is the majority of cases of lung cancer and continues to be one of the deadliest cancers world...