F, prostatic fluid sample:Prostatic fluid specimen after prostate massage by the acquisition, directly drop when less liquid on a glass slide and timely submission shall be taken to prevent sample evaporation to dryness, the amount collected for a long time in a clean, dry test tube. If massag...
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The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IGF-1. in the samples is then determined by comparing the O.D. of the samples to the ...
we found that CLD3-CBP3 cannot bind to both ALS and IGF1 (Fig.5b), suggesting that NBP3 is required for IGF1/IGFBP3 binary complex formation. Unfortunately, we could not test whether CBP3 alone is sufficient for ALS binding, because we were unable to express CBP3 probably due to the...
(5 ml). The homogenate was then placed in a 40 ml ultracentrifuge tube and overlaid with 10 ml 1 M sucrose, 0.1 mM CaCl2. The gradients were centrifuged at 100,000 g for 3 hrs. The synaptosomal fraction (4–5 ml) was collected at the 1.25 M/1 M ...
However, the migration, tube formation, proliferation and expression of various genes involved in the angiogenesis (VEGF, VEGFR-1, bFGF, PDGF-A and PDGF-B) of human umbilical VE cells (HUVECs) remained unchanged after the drug treatment (Fig. 4a and Supplementary Fig. 6). Our next step ...
hallmark epithelial-mesenchymal transition, tube morphogenesis, growth, and secretory granule lumen (Supplementary Fig. 4E). Subsequently, we identified significant receptor-ligand interactions between different cell types using CellPhoneDB. Compared to IGF2BP3-SPHK1- BLCA, IGF2BP3-SPHK1 + BLCA,...
C-Cbl ubiquitinates IGF-1-activated IGF-1R at K48 (Sehat et al., 2008). Since the binding between c-Cbl and IGF-1R is PrP dependent (Figure 3F), IGF-1R ubiquitination may also be PrP dependent. To test this hypothesis, we first quantified IGF-1R mRNA from melanoma cells with or wi...
The animals were fasted for 24h before the end of the experiment. For Rex rabbits at 150 days of age, 5 mL blood samples were placed into sterile vacuum coagulation vessels and centrifuged at 3000×gfor 10 min after coagulation. Upper serum was collected, transferred to a sterile tube, and...
Color Black Red Blue Green Product Name Refrigerant Charging Hoses Certtification RoHS, ISO9001 Size Range 3.2- 25.4 mm Features UV & Ozone Resistant Working Pressure 600 Psi Burst Pressure 3000 Psi Construction Tube/Reinforcement/Cover Ozone R...