Mean forward scatter signal intensity was slightly higher in NP naïve B cells (70,724 MFI) compared to resting blood naïve B cells (64,560 MFI) from the same subject (p value 0.0108, paired t-test, two tailed) (Supplementary Fig. 3c). NP IgD+ B cells can be mutated Direct or ...
Extended-release systems include any dosage form that maintains therapeutic blood or tissue levels of the drug for a prolonged period. If the system can provide some actual therapeutic control, whether this is temporal or spatial or both, of drug release in the body, it is considered a controll...
Typically, a polypeptide of the invention is able to remove IgE bound to the surface of a basophil or a mast cell in human blood. The polypeptide may remove IgE from more than 50% of the basophils and/or mast cells in a sample of human blood, preferably more than 55%, 60%, 65%, ...
Blood samples were obtained by the puncture of the right ventricle of immunized mice. ELISA for detection of Abs was performed as described [25]. Plasma was tested for IgG1 or IgG2a Abs using venom-coated 96-well plates and biotinylated goat anti-mouse IgG1 or IgG2a antiserum. The reactions...
(A) Representative flow cytometry of blood B cells from homozygous Verigem mice (see also Figure S1), or a wild-type control, 4 days after in vitro stimulation with anti-CD40 and IL-4. n = 3. (B and C) Flow cytometry of cells from the draining LNs of homozygous Verigem mice 8 da...
active complement, but the activity declines during storage and handling. Accordingly, the complement activity may vary between different patients and also between different samples from the same patient. To avoid activation of the complement cascade, EDTA is often included in tubes used for blood ...
In the present study we have examined the relationship between serum IgE and surface IgE and FcϵRIα and FcϵRII expression for human blood basophils across a 6-log range of IgE levels, a much larger range of IgE levels than previously studied. Furthermore, we have examined the ...
Gal-9 expression has been shown in human cord blood-derived mast cells (20), but the function of Gal-9 in mast cells has not been elucidated yet, although an effect of TIM3 activation in mast cells was demonstrated to augment Th2 cytokine production using a polyclonal anti-TIM3 antibody,...
Blood, on the other hand, contains metabolically active compounds, and lipid-soluble metabolites that are not present in urine. Several studies have identified altered metabolic pathways in association with asthma [5,6]. However, an integrative analysis of metabolic responses across blood and urine ...