Analytes added to the sample well dissolved the labeled antibody (tracer), and the antigen–antibody complex formed was transported by the flow caused by capillary action to the test line. The color signal of the test line was in proportion to the morphine concentration in urine samples. Based...
and select a proper dilution factor to make the diluted target protein concentration fall in the optimal detection range of the kit. Dilute the sample with the provided dilution buffer, and several trials may be necessary. The test sample must be well mixed with the dilution buffer. And also ...
and select a proper dilution factor to make the diluted target protein concentration fall in the optimal detection range of the kit. Dilute the sample with the provided dilution buffer, and several trials may be necessary. The test sample must be well mixed with the dilution buffer. And also ...
METHOD OF MESURING CONCENTRATION AND/OR RELATIVE CONTENT OF SPECIFIC IMMUNOGLOBULIN IN A TEST TUBEA method of detecting an antibody in a sample using a labelling compound and comprising the steps of mixing a ligand antigen, antibody or hapten bound to biotin with the sample; an antibody directed...
An equal volume of (200 μL) color reagent was added to each tube. These tubes were kept in water baths at 38°C for 4 hours. After this incubation, all the test tubes were cooled in an ice bath and treated with 1 mL of 12 M H2SO4 with constant stirring. As a result, ...
we could express functional MIB-MIP gene tandems individually to test their activity and we also attempted expression of heterologous MIB-MIP systems from otherMollicutesspecies, proving thatMferiis a valuable bacterial platform for functional genomics studies of this and other species. Finally, we als...
To test for differences in epitope competition levels, we used an unpaired, two-tailed t-test (Graphpad Prism version 8.3.1). To test for difference in V gene usage between groups, we conducted a Kruskal–Wallis test using ‘kruskal.test‘ in R version 4.0.3. This was followed by a Dun...
Clinical and molecular features were compared using the Fisher exact test and unpaired (2-tailed) t-test. Overall survival (OS) and progression-free survival (PFS) were calculated from the date of diagnosis to the date of last follow-up or death and to the date of disease progression or de...
Abstract Ro52/TRIM21 plays a key role in antibody-dependent pathogen neutralization and is a major autoantigen in systemic lupus erythematosus, Sjögren’s syndrome (SS), and other autoimmune diseases. Here we evaluated immunoreactivity against Ro52-related molecules in SS and healthy volunteers. Al...
Briefly, 10 µmol of total lipids (DOTAP:DC-chol at 1:1 mol ratios) was mixed in a glass tube and evaporated to dryness in vacuo. Retained lipid films were hydrated by adding 250 µL of PBS and vortexing for 5 min. Multilamellar vesicles were then extruded 10 times through a 100...