METHOD RELATING TO SELECTION OF CDNA CLONE ENCODING PROTEIN OF C-TERMINAL REGION OF HYBRIDOMA AND ANTIGEN PRODUCING P-GLYCOPROTEIN CELL SURFACE ANTIGEN SPECIFIC ANTIBODYThe invention provides a method for selecting hybridomas which produce antibodies specific to domains of a cell surface antigen which ...
methods as an alternative is further enhanced by the ability to precisely control and manipulate selection conditions for antibodies-specific traits. The flexibility of display methods also helps to overcome the difficulty of generating anti-self antibodies due to tolerance. This is possible because the...
Combinatorial libraries are preferred for binding site isolation, as they incorporate selection methods and assays for sFv or Fab that detect only properly refolded molecules, as phage antibodies and as secreted sFv or Fab. Table VII. VH Ammo-Terminal Sequences Used for 741F8 sFv'a Source...
Hybridomas, fusions of primary mouse B cells and myelomas, are stable, rapidly-proliferating cell lines widely utilized for antibody screening and production. Antibody specificity of a hybridoma clone is determined by the immunoglobulin sequence of the p
This means several months and up to 1 year are typically required before the selection of an optimal stable clone10. Therefore, stable cell line generation is typically out of practical reach for academic and small-to-medium-sized entities. A method to reduce the effort and time taken to ...
When an antigen reacts with B-lymphocyte receptors, lymphocytes divide rapidly and produce a clone of B cells, all these B cells produce antibodies against that specific antigen and this is called as clonal selection. That is B-lymphocytes produce only one type of antibodies which are specific ...
methods as an alternative is further enhanced by the ability to precisely control and manipulate selection conditions for antibodies-specific traits. The flexibility of display methods also helps to overcome the difficulty of generating anti-self antibodies due to tolerance. This is possible because the...
The final step is to ensure that all cells in the culture really are a clonal population. Cells are usually diluted so that only one cell is deposited into a culture vessel. The single cell divides, producing a clone of identical hybridoma cells. The antibody produced by these cells is a ...
A clone, N12-16... K Kitano,K Iwamoto,Y Shintani,... - 《Journal of Immunological Methods》 被引量: 24发表: 1988年 Enhanced antibody production of human-human hybridomas by retinoic acid The enhancement of human monoclonal antibody production by retinoic acid (RA) was evaluated usingthe hum...
Supernatants from hybridoma SK 7 had no effect on the synthesis of interleukin 2 by a clone of the Jurkat human T cell leukemia line, on the utilization of this lymphokine by an interleukin 2 dependent T cell clone, or on peroxide-mediated macrophage cytotoxicity. It is improbable that the...