. As a result, a hybridoma has the immortality and production capacity of the myeloma but the knownantibody specificityof the B cell. The hybridoma grows to form a colony secreting large quantities ofmonoclonal antibody(mAb); that is, antibody derived from a single, defined B cell clone....
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Hybridomas, fusions of primary mouse B cells and myelomas, are stable, rapidly-proliferating cell lines widely utilized for antibody screening and production. Antibody specificity of a hybridoma clone is determined by the immunoglobulin sequence of the p
(Supplementary Fig.5b). To clone theIGHlocus into the IGK-NAC, FRT/FLP recombination-mediated reciprocal translocation between the IGK-NAC and modified hChr.14 was performed in CHOHprt-/-cells and HPRT gene reconstruction with the desired product again enabled selection of CHO cells that carried...
α1,6-FucT is encoded by the fut8 gene and different strategies targeting fut8 or other fucose-related genes have been developed to reduce the fucosylation capacity of recombinant mAb producing cells. The fut8 gene [19], the GMD gene [20], or both [21] have been knockdown in CHO cells...
andclonallyexpandedafterselection.Theassayhasbeenusedtoreclone raresecretorsfromhybridomaculturesandtoenhancetheproduction ofcultureswithpoorproducers.Theassayis easilygeneralized forthedetection ofanysecretedprotein forwhichspecificantibodies orotherligandsare ...
However, unlike a normal plasma cell clone that dies after a few days, a myeloma clone has an unlimited life span: it is said to be “immortal.” This means that unlimited numbers of cells producing massive amounts of a single antibody can be grown in culture and manipulated in the ...
A combination of these strategies can be applied to our PnP platform and thus may result in greater HDR efficiency, which would further simplify selection. Higher HDR efficiency raises the possibility of eventually incorporating antibody or protein libraries into PnP cells, which could be screened by...
(de St Groth and Scheidegger, 1980). After selection, individual hybridoma clones are separated so that each clone produces a singlemonoclonal antibodywith one specificity in a process known as subcloning. Antibodies from individual clones are then assayed for binding to the immunization antigen. ...
(5 plates from each donor). After 14 days in HAT selection, the hybridomas were screened for IgA reactivity with type 3 Sabin PV by immunocapture ELISA. We identified a total of six clones, one clone (1E4) from donor P3 and five clones (1A12, 1H10, 2A6, 2D10 and 5F10) from ...