However, unlike a normal plasma cell clone that dies after a few days, a myeloma clone has an unlimited life span: it is said to be “immortal.” This means that unlimited numbers of cells producing massive amounts of a single antibody can be grown in culture and manipulated in the ...
(de St Groth and Scheidegger, 1980). After selection, individual hybridoma clones are separated so that each clone produces a singlemonoclonal antibodywith one specificity in a process known as subcloning. Antibodies from individual clones are then assayed for binding to the immunization antigen. ...
The antibodies are used to obtain a cDNA probe which in turn was used to select a cDNA clone encoding for a portion of the p-glycoprotein including the C-terminal end. The C-terminal portion of the P-glycoprotein comprising about 239 amino acids and localized to the cytoplasmic side of ...
Equine IL-6 activity can be detected using the murine hybridoma cell line B 13.29 clone B.9 (Morris et al., 1992). Equine IL-6 cross-reacts with antibodies to human IL-6 in neutralization and slot-blot radioimmunoassays (Billinghurst et al., 1995). The cDNA for equine IL-6 has recen...
This means several months and up to 1 year are typically required before the selection of an optimal stable clone10. Therefore, stable cell line generation is typically out of practical reach for academic and small-to-medium-sized entities. A method to reduce the effort and time taken to ...
Hybridomas, fusions of primary mouse B cells and myelomas, are stable, rapidly-proliferating cell lines widely utilized for antibody screening and production. Antibody specificity of a hybridoma clone is determined by the immunoglobulin sequence of the p
When an antigen reacts with B-lymphocyte receptors, lymphocytes divide rapidly and produce a clone of B cells, all these B cells produce antibodies against that specific antigen and this is called as clonal selection. That is B-lymphocytes produce only one type of antibodies which are specific ...
Selection of an appropriate B cell is an important aspect of this invention and any proposed cell line should be tested to confirm that the B cell receptor signaling pathway is fully functional. Individual B cell clones having the aequorin gene should be tested to identify a particular clone wit...
Selection of MR-positive 43 cells MR-positive 43 cells (43MR) were selected as described below. Upon initial analysis the parental 43 cells were negative for MR expression. For induction of MR expression, cells were cultured in an equal mix of fresh and spent/conditioned media (RPMI 1640 plu...
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