Kinetic analysis of antibodies is crucial in both clone selection and characterization. Historically, antibodies in supernatants from hybridomas are selected based on a solid-phase enzyme-linked immunosorbent assay (ELISA) in which the antigen is immobilized on the assay plate. ELISA selects clones ...
(de St Groth and Scheidegger, 1980). After selection, individual hybridoma clones are separated so that each clone produces a singlemonoclonal antibodywith one specificity in a process known as subcloning. Antibodies from individual clones are then assayed for binding to the immunization antigen. ...
However, unlike a normal plasma cell clone that dies after a few days, a myeloma clone has an unlimited life span: it is said to be “immortal.” This means that unlimited numbers of cells producing massive amounts of a single antibody can be grown in culture and manipulated in the ...
Using agarase, the cells can be recovered from the microdroplets and clonally expanded after selection. The assay has been used to reclone rare secretors from hybridoma cultures and to enhance the production of cultures with poor producers. The assay is easily generalized for the detection of any...
When an antigen reacts with B-lymphocyte receptors, lymphocytes divide rapidly and produce a clone of B cells, all these B cells produce antibodies against that specific antigen and this is called as clonal selection. That is B-lymphocytes produce only one type of antibodies which are specific ...
(Supplementary Fig.5b). To clone theIGHlocus into the IGK-NAC, FRT/FLP recombination-mediated reciprocal translocation between the IGK-NAC and modified hChr.14 was performed in CHOHprt-/-cells and HPRT gene reconstruction with the desired product again enabled selection of CHO cells that carried...
Only one study has shown that in human ovarian cancer cells SK-OV-3, luciferase reporter assay indicated that the 1 kbp 5'-flanking region of exon E1 conferred the promoter activity. This region contains a TATA-box, but not a CCAAT motif, and potential binding sites for some transcription ...
Effective production of a human monoclonal antibody against tetanus toxoid by selection of high productivity clones of a heterohybridoma. A mouse路human-human heterohybridoma, N12-16.63, has been described which produces an antitetanus toxoid human monoclonal antibody (MoAb). A clone, N12-16... K...
The present monoclonal antibody was produced by using the cell obtained from selection of hybridomas produced according to the so called cell fusion method. More specifically, a fused hybridoma was formed between antibody-producing cells and myeloma cells, said hybridoma was cloned and a clone capabl...
the mutagenized human T cell lines are not able to grow, and only the hybridoma cells are able to grow, i.e., are HAT resistant. The isolated T cells would also not survive since they do not have the ability for constitutive growth. In this manner, selection of hybridomas can be achi...