As the time to sequence the entire human genome approaches, it appears that the predominant method of collecting this data will entail some variation of the shotgun sequencing strategy [1]. The startpoint for the basic shotgun experiment is a pure sample of a large number of copies of a ...
W. Human whole-genome shotgun sequencing. Genome Res. 7, 401–409 (1997). Article CAS PubMed Google Scholar Green, P. Against a whole-genome shotgun. Genome Res. 7, 410–417 (1997). Article CAS PubMed Google Scholar Venter, J. C. et al. Shotgun sequencing of the human genome...
accuracy approaching that of short-read sequencing technologies, these platforms have proven their ability to resolve some of the most challenging regions of the human genome, detect previously inaccessible structural variants and generate some of the first telomere-to-telomere assemblies of whole ...
For each individual, we extracted genomic DNA from either whole blood or saliva samples, as available. We fragmented DNA, prepared and sequenced shotgun-sequencing libraries using Illumina or BGI kits following manufacturer’s instructions in either paired-end 100-bp (case 4) or 150-bp read ...
An SNP map of the human genome generated by reduced representation shotgun sequencing. Describes a method called reduced representation shotgun sequencing, for the creation of maps of single nucleotide polymorphisms (SNPs). The method, which ... Altshuler,David,Pollara,... - 《Nature》 被引量...
The human gut is inhabited by a complex and metabolically active microbial ecosystem. While many studies focused on the effect of individual microbial taxa on human health, their overall metabolic potential has been under-explored. Using whole-metagenome shotgun sequencing data in 1,004 twins, we ...
摘要: A mixed 'clone-by-clone' and 'whole-genome shotgun' strategy will be used to determine the genomic sequence of the . This method will allow a phase of rapid annotation of the contemporaneous sequence draft, through whole-genome 'sample sequence comparisons'....
Human whole-genome shotgun sequencing. Genome Res. 1997; 7 :401–409.Weber JL, Myers EW. Human whole-genome shotgun sequencing. Genome Res. 1997; 7 :401–9.Weber, J.L. and Myers, E.W. 1997. Human whole-genome shotgun sequencing. Genome Res. 7 : 401-409....
(marker gene) sequencing, a method that amplifies variable regions of a highly conserved bacterial gene, such as the 16S rRNA gene, enabling to infer taxonomic/genomic relationships based on their phylogeny; and (2) whole genome shotgun sequencing or metagenomics, an approach that sequences all ...
Each dataset contained whole genome shotgun sequence reads of metagenomes obtained from fecal samples. Two sets allowed the possibility of comparing the presence of L. paracasei strains in the microbiomes of healthy individuals and patients with chronic disease (children with autism spectrum disorders ...