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DEPTOR-m TOR signaling is critical for lipid metabolism and inflammation homeostasis of lymphocytes in human PBMC culture. Xie Q B,Liang Y,Yang M,et al. Journal of Immunological Research . 2017Xie Q B,Liang Y,Yang M,et al.DEPTOR-mTOR sig-naling is critical for lipid metabolism and ...
The impact of M-CSF and RANKL in the common OC co-culture was assessed for OB, FB and OK via MTT assay via DAPI control. The multinuclearity and function of OC were evaluated by light microscopy, DAPI staining, resorption assay and FACS analysis. The PBMC showed the highest differentiation...
样品类型细胞培养,PBMC 运输条件室温 目标种属人 Unit SizeEach 内容与储存 本产品含:Dynabeads™ CD3⁄CD28 以悬浮液形式提供,浓度为 1 x 108个微珠 ⁄mL 磷酸盐缓冲盐水 (PBS),pH 7.4,含有 0.1% 人血清白蛋白 (HSA)。 储存于:2-8 °C
Human embryonic kidney 293 (HEK293) cells were maintained in suspension culture with Freestyle medium (Gibco 12338018) supplemented by 1% heat-inactivated fetal bovine serum (Sigma F2442) at 37 °C, 5% carbon dioxide and 70% humidity as previously described46. For all transient transfections,...
Freshly isolated, allogenic PBMCs were irradiated with a dose of 50 Gy and added to the sterile-filtered culture medium at a concentration of 2.5x105 cells/mL. After FACS sorting and counting, B cells were diluted in stimulation medium to a concentration of 20-80 cells/mL, seeded in 384-...
Feeder-Free Culture & Passaging of Human iPS Cells Using Complete KnockOut™ SR XenoFree Feeder-Free Medium Reprogramming Peripheral Blood Mononuclear Cells (PBMCs) Reprogramming Fibroblasts with the CytoTune®-iPS Sendai Reprogramming Kit Culturing Pluripotent Stem C...
Conditioning of human embryonic stem cell-derived endothelial cells with PBMCs confers TLR4 sensing in co-culture conditionsHuman embryonic stem cell-derived endothelial cells (hESC-EC) are being investigated as potential therapies for vascular disease. We have previously shown that hESC-EC do not ...
文件中指明生产CAR-T细胞产品的原材料是指其生产过程中所用的所有生物原材料和化学原材料,它们不是CAR-T细胞产品的目标组成成分,如培养基、PBMC分离试剂、T细胞分选试剂、激活剂、细胞因子(如IL-2、IL-7及IL-15)、血清或血清替代物等。 2022年1月6日,国家药监局发布公开征求《药品生产质量管理规范-细胞治疗产品...
Culture of human primary osteoclasts Human OCs were prepared as reported by Mitsuzaki et al. [52] with slight modifications. Peripheral blood was collected from healthy normal volunteers after informed consent. Mononuclear cells (PBMCs) were prepared from diluted peripheral blood (1:2 in Hanks Bala...