Explore Precision for Medicine’s extensively characterized human PBMCs, processed in an FDA-registered facility for optimal quality. Learn more today.
样品类型细胞培养,PBMC 运输条件室温 目标种属人 Unit SizeEach 内容与储存 本产品含:Dynabeads™ CD3⁄CD28 以悬浮液形式提供,浓度为 1 x 108个微珠 ⁄mL 磷酸盐缓冲盐水 (PBS),pH 7.4,含有 0.1% 人血清白蛋白 (HSA)。 储存于:2-8 °C
Freshly isolated, allogenic PBMCs were irradiated with a dose of 50 Gy and added to the sterile-filtered culture medium at a concentration of 2.5x105 cells/mL. After FACS sorting and counting, B cells were diluted in stimulation medium to a concentration of 20-80 cells/mL, seeded in 384-...
Human pluripotent stem-cell-derived islets (hPSC-islets) are a promising cell resource for diabetes treatment1,2. However, this therapeutic strategy has not been systematically assessed in large animal models physiologically similar to humans, such as no
(1:1 PBMC to Treg ratio). The therapeutic antibodies were added, and after 3 days of culture, cytokine levels were measured in the culture supernatant using ProcartaPlex immunoassay according to the manufacturer’s recommendations (Th1/Th2/Th9/Th17/Th22/Treg Cytokine 18-Plex Human Procarta...
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whole-blood samples collected from consented patients were diluted with PBS, and PBMCs were separated using standard Ficoll Paque density gradient centrifugation method. Following the PBMC separation, cells were cultured in expansion media containing StemSpan H3000, stem cell factor, interleukin-3, eryth...
isolated from patient 2 showed reduced MLKL protein levels, accompanied by a nominal increase in pro-inflammatory cytokine production in response to the Toll-like receptor (TLR) agonists, lipopolysaccharide and poly I:C, relative to age and sex matched healthy donor PBMCs not carrying theMLKL S13...
Culture of pluripotent stem cells and generation of skeletal progenitors For all experiments, research grade ESI-017 were used; the stock line was purchased from BioTime. Cells were cultured on hESC-qualified, LDEV-free Matrigel (Corning # 354277) in mTesR1/Plus media and passaged using ReLeSR...
(Miltenyi Biotech) following isolation of PBMC by standard Ficoll density gradient centrifugation9,50. Freshly isolated blood CD4+T cells were stained with CFSE (Cell Division Tracker Kit; BioLegend) as recommended by the manufacturer. The cells were then resuspended in immune cell media as ...