5. Xu, H., et al.,Influence of various medium environment to in vitro human T cell culture.In Vitro Cellular & Developmental Biology. Animal, 2018.54(8): p. 559-566. 6. Sato, K., et al.,Impact of culture medium on the expa...
Assessing human B cell responses to influenza virus vaccines and adjuvants in a PBMC-derived in vitro culture systemIn vitro systems based on human peripheral blood mononuclear cells (PBMCs) can bridge the gap between preclinical and clinical vaccine evaluation but have so far mainly been exploited...
57-64.5. Caeser, R., et al.,Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells. Nature Protocols, 2021. 16(5): p. 2499-2519.6. Kuo, T.C., et al., Repression of BCL-6 is required for the formation of human memory B cells in vitro. ...
PBMCs from healthy human donors (N = 18) were cultured for 3 days with nivolumab or an isotype control antibody (20 μg/mL) at the initiation of the assay together with serial dilutions of SEB. Interleukin-2(IL-2) levels in culture supernatants were measured by ELISA analysis. Antigen-s...
3. Toda, G., et al.,Preparation and culture of bone marrow-derived macrophages from mice for functional analysis.STAR Protocols, 2021.2(1): p. 100246. 4. Davies, J.Q. and S. Gordon,Isolation and culture of mu...
The HIV-1 gag p24 content was determined at seven days post-infection in the culture supernatant using the HIV-1 p24 antigen ELISA kit (Biomerieux). Mock-infected PBMCs were used as the negative con- trol. The experiments using other laboratory strains and viral isolates were performed as ...
Commonly, T cells are seeded at a final concentration of 1–2.5 × 106 cells/mL in 24 or 48-well cell culture plates (Pascal et al. 2013). Although, in T cell proliferation ([H3]-thymidine) and viability (MTT or XTT) assays, 96-well plates are used and only 100 μL of the...
They were plated with human PBMCs thawed overnight in culture media supplemented with human AB serum and a cocktail of cytokines (IL-1b, IL-2, IL-4, IL-6, and IFN纬). On days 7 and 14, cells were harvested and analyzed for proliferation of antigen-specific cells. After fourteen days...
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This chapter describes the main applications, isolation techniques, and culture conditions for PBMC-derived T cells. Furthermore, critical parameters of the model, together with the experimental read outs will be discussed. Copyright 2015, The Author(s). 展开 ...