Most previous studies focused on one particular mechanism or cell type, leaving a gap in our understanding of muscle aging as a whole. To address this, recent mouse and human skeletal muscle studies pioneered the use of either single-cell RNA sequencing (scRNA-seq)12,13,14,15,16or single-n...
Taken together, this RNA-seq analysis of PCD temporal stages helped identify coexpressed gene modules with associated up/downregulated transcription regulator genes that could represent major regulatory nodes for human PC maturation. These data constitute a unique resource of human PCD gene expression ...
Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo. On the other ha
When HIV infects CD4 cells, it converts its RNA to DNA using its HIV reverse transcriptase enzyme. It then inserts HIV DNA into the cell’s human DNA. The human-like DNA of HIV then becomes part of the infected person's own cells, hijacking the cell to produce more HIV virus. NRTIs...
What common pathway was identified between Single Cell Rna Sequencing (scRNA-seq) data of old versus young human skin and RNA-seq data from fibroblasts with HES1 knockdown? What types of skin cells showed decreased density during aging?
Next, we integrated iMG sc-RNA-seq data with the post-mortem single-nucleus RNA-seq (snRNA-seq) data [48] of aging frontal cortex from the Religious Orders Study and Rush Memory and Aging Project (ROSMAP) donors [30,49,50]. snRNA-Seq data from ROSMAP donors defined cellular types in...
and not exclusively expressed in non-endocrine cells according to single cell RNA-seq. We allowed for inflation of ≤ 5 interactors, and used default (>0.4) confidence scores. Shown are two networks with >3 components, one populated by components of heterotrimeric G protein signaling, and anothe...
The scRNA-seq libraries were constructed using the Single-cell RNA Library Kit (Singleron Biotechnologies, Nanjing, China). The constructed libraries were pooled onto an Illumina HiSeq × 10 sequencer machine for sequencing. Raw reads were processed by the CeleScope (https://github.com/...
RNA-seq reads were trimmed to a maximal length of 80 bp and stretches of bases having a quality score <30 at the ends of the reads were removed. Reads were mapped using Tophat 2.0.6 [19]. As reference sequence for the transcriptome mapping we used the current assembly of the human gen...
Single-cell RNA-seq (scRNA-seq) provides the opportunity to generate the detailed transcriptomic cell profiling required to address the drawbacks of bulk RNA-seq [6,7,8]. However, performing scRNA-seq requires cell re-suspension, and library preparation needs to be done from fresh tissue. This...