Objective: To study the effects of human adipose derived mesenchymal stem cell conditioned medium (ASC-CM) on the viability, apoptosis of HT29 cells of colon cancer. Method: The cultured HT29 cells with good condition in vitro were divided into two groups, one group with the ASC-CM culture...
The replacement of glucose by galactose in the culture medium resulted in partial structural and functional enterocytic differentiation of HT29 cells. In o... J Fantini,B Abadie,A Tirard,... - 《Journal of Cell Science》 被引量: 248发表: 1986年 ...
REPORTER ASSAYS Cell preparation 1. Rinse cell layer with PBS and detach cells with trypsin. 2. Centrifuge cells at 300 x g (RCF) for 5 minutes. 3. Remove supernatant and resuspend cells at 2.8 x 105 cells/ml in test medium (DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10%...
Subculture Routine:Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.05% trypsin/EDTA; 7.5-10% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm². Culture Medium:DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS). ...
After 1 month of daily addition of 10 U/ml adenosine deaminase to the culture medium, HT29 cells were cloned by limited dilution. Among the clones obtained, we focused our attention on clone 13. Microscopic visualization and proliferation studies indicated that cells from this clone grew very ...
1. 1. Carbohydrate metabolism was studied in HT29 human colon cancer cells cultured in a glucose free medium supplemented with 2.8 mM inosine (HT29ino cells) in comparison with standard HT29 cells grown in the permanent presence of glucose (HT29Glc + cells) and with HT29Glc— cells which...
Objective: To study the effects of human adipose derived mesenchymal stem cell conditioned medium (ASC-CM) on the viability, apoptosis of HT29 cells of colon cancer. Method: The cultured HT29 cells with good condition in vitro were divided into two groups, one group with the ASC-CM culture...
In the absence of sucrose, the radioactivity associated with the cells reached a plateau value within 20 min. Removal of surface-bound radioactivity by acid–NaCl wash revealed that about 80% of the total 125I-Tyr3-NT bound was intracellular (Fig. 1). In the presence Discussion The present...
China 【Abstract 】 Objective To investigate the difference of tumor stem cells in HT29 and HCT116 cell lines and evaluate the model of colon cancer stem cell research. Methods HT29 and HCT116 cells were cultured in serum-free medium (SFM)to develop spheres. The growth states of spheres in...
【摘要】Objective To investigate the role of cancer stem cells(CSC)in the acquired resistance to Oxaliplatin (L-OHP)of human colon cancer cell line HT29.Methods L-OHP-resistant human colon cancer cell line HT29 (HT29/L-OHP)was established in vitro by exposure to increased L-OHP ...