To count positive cells in the hippocampus, three fields per brain section were eval- uated under 20X magnification of a light microscope and all immunopositive cells were counted. The same slide contained control and experimental sections. Counts are expressed as the average cell number per field...
In both the BLA and PlCo, all the GFP-positive cells also expressed NeuN (Fig. 16a,b). Since DAPI is a nuclear marker, the GFP was colocalized with DAPI in cells with small nuclei (Supplemental Fig. 3C). On the other hand, the subcellular localizations of GFAP, APC, and CD11b were...
1Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. 2Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China ...
In GFAP-HSV-TK mice, proliferating GFAP-positive cells that express the HSV-TK transgene metabolize the antiviral compound ganciclovir ACNP 49th Annual Conference (GCV) to toxic nucleotide analogues which induces cell death. As adult neural stem cells are derived from astrocytes expressing GFAP, ...
Thereby, the proportions of differentiated cell types within colonies grown during exposure to serotonin-targeting drugs can be assessed by immunohistochemical staining for markers of neurons (Peripherin), glia (GFAP), and myofibroblasts (SMA) with multipotent colonies identified by the presence of all...
It has been shown that EGFR plays a critical role in glial cell survival during embryonic development [103]. In addition, EGFR knock-out mice contain lower numbers of GFAP positive astrocytes in cortex and develop progressive neurodegeneration in the frontal cortex, olfactory bulb and thalamus [...