Areas positive for HSV-1 were observed in the medulla of brains from both WT and STING-deficient mice, although we observed a larger number of HSV-1-infected cells in the sections from the STING-deficient mice (Fig. 1j–l). These data are consistent with HSV-1 entering into the CNS ...
Distribution of anti-HSV-1 IgG positive and negative prevalence in Bororó (right) and Jaguapirú (left) villages (Dourados, Mato Grosso do Sul (MS), Brazil) Full size image The prevalence of anti-HSV-1 IgG in Indigenous people who declared to be retired was 97%, lived in another villag...
Infection is most accurately diagnosed by IgG serology: patients remain seropositive for life. The prevalence of HSV-1 infection is about 70% in diverse human populations. There is a great spectrum in the severity of HSV infections. Only a minority of persons with HSV corneal infection progress...
Syndecan-1, an HSPG previously linked to HSV-1 infection43,44, generated a positive PLA signal in the presence of gC (Fig. 3b), confirming that this HSPG co-localized with gC. MARCO also generated a strong positive PLA signal (Fig. 3a), indicating that MARCO and gC are residing on ...
Mouse polyclonal anti-IgG Proteintech Cat# B900620; RRID: AB_2883054 Rabbit monoclonal anti-N6-Methyladenosine Cell Signaling Technology Cat# 56593;RRID: AB_2799515 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076;RRID: AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell ...
Alexa Fluor 555-conjugated donkey anti-mouse immunoglobulin G (IgG) (1:2,000, Life Technologies) antibody containing 10 μg/mL Hoechst 33342 in PBS (Invitrogen, H3570) was used to visualize the primary antibody and label the nuclei. Cell Viability Assay Vero cells were harvested in a 1.5-...
added and included goat anti-rat IgG conjugated with Alexa Fluor 546 (Invitrogen, Eugene, OR), donkey anti-rabbit Alexa Fluor 488 (Jackson ImmunoResearch, West Grove, PA), goat anti-hamster TRITC (Jackson ImmunoResearch) all diluted 1:200 in 0.1% Triton/PBS and incubated overnight at 4 °...
The cells were incubated with a mouse antibody to US11 (gift from Dr Roizman) at 4 1C overnight, washed three times for 5 min in TBS with 0.05% TWEEN 20, incubated with a FITC conjugated-Goat anti-Mouse IgG antibody for 1 h at RT (1:64, Sigma, Cat# F2012, St Louis, MO, ...
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
Positive samples were titrated over time after 12 days. In 90 study participants, there was a fourfold or greater increase in IgG antibody titers, as well as the absence (negative test) of IgM and G to HSV-2, which can serve as a criterion for the current disease. The absence of IgM ...