cells in green are HSV-1+with DAPI staining in blue. (B) Metamorph quantification of the cornea area positive for HSV-1 antigen. Horizontal bars depict mean of percentage threshold area positive for anti-HSV-1 positive spots ± SEM (*p < 0.05 comparing the two groups as determined...
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
Alexa Fluor 555-conjugated donkey anti-mouse immunoglobulin G (IgG) (1:2,000, Life Technologies) antibody containing 10 μg/mL Hoechst 33342 in PBS (Invitrogen, H3570) was used to visualize the primary antibody and label the nuclei. Cell Viability Assay Vero cells were harvested in a 1.5-...
We pre-treated protein A/G magnetic beads (MCE, NJ) with apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) antibody (Santa Cruz Biotechnology, Dallas, TX) or mouse IgG (CST, Danvers, MA) to form bead–antibody complexes. The vulvar tissues were homogenized ...
The cells were incubated with a mouse antibody to US11 (gift from Dr Roizman) at 4 1C overnight, washed three times for 5 min in TBS with 0.05% TWEEN 20, incubated with a FITC conjugated-Goat anti-Mouse IgG antibody for 1 h at RT (1:64, Sigma, Cat# F2012, St Louis, MO, ...
Finally, we should acknowledge that we cannot completely exclude novel infection/cross-transmission of HSV-1 instead of reactivation, as we have serological HSV-1 status at admission only for 12 patients (all with positive IgG). However, since all of them were positive for previous infection, ...