cells in green are HSV-1+with DAPI staining in blue. (B) Metamorph quantification of the cornea area positive for HSV-1 antigen. Horizontal bars depict mean of percentage threshold area positive for anti-HSV-1 positive spots ± SEM (*p < 0.05 comparing the two groups as determined...
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Comparison of means was achieved using Student's t-test and one- way analysis of variance where appropriate with a significance assigned at Po0.05. Quantitative RT-PCR data were compared using analysis of variance and unpaired t-tests. CONFLICT OF INTEREST Drs Skelly and Weichselbaum are co-...
To further confirm the infectivity of HSV-1 in Cas9/gRNA-expressing cells, we used ICP5, one of the major capsid proteins, to visualize HSV-1-infected cells. Using immunofluorescence, clear reduction of ICP5-positive cells was found in SpUL8 (10.73% ± 7.90%) and SpUL52 (36.68% ± ...
Herpes Simplex Virus Type 2 (HSV-2) is one of the most common sexually transmitted diseases. Although there is still no licensed vaccine for HSV-2, a theoretical investigation of the potential effects of a vaccine is considered important and has recently
Indeed, in vitro assays have shown that ZIKV can infect cytotrophoblasts and invasive extravillous trophoblasts by means of cell-to-cell transmission after initial viral replication in the uterine wall; however, SYNs are resistant (El Costa et al., 2016; Weisblum et al., 2017), as proven...
We pre-treated protein A/G magnetic beads (MCE, NJ) with apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) antibody (Santa Cruz Biotechnology, Dallas, TX) or mouse IgG (CST, Danvers, MA) to form bead–antibody complexes. The vulvar tissues were homogenized ...
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
Patterns of expression differ slightly in the adult: NT-3-specific hybridization was limited only to inner hair cells, which are innervated by TrkC-positive, Type I SGNs (4, 14, 15). Type I SGNs are the primary auditory afferent neurons that deliver sound signal information from inner hair ...
Of these 13 patients tested for HSV-1-DNA on blood, 12 had HSV-1 reactivation on BALF, while 1 had no HSV-1 reactivation on BALF. Blood HSV-1-DNA was positive in 3/13 patients. All the three patients with positive HSV-1-DNA on blood had HSV-1 reactivation detected on BALF samples...