HSV-TK基因丙氨基鳥苷旁觀者效應 HSV-TK geneGCVBystander effect目的觀察單純皰疹病毒胸昔激酶基因HSV-TK/GCV系統對人肺癌細胞H446的體外殺傷作用.方法 利用脂質體(lipofectin)將重組逆轉錄病毒載體pLXSN-TK導人包裝細胞PA317,G418篩選至抗性克隆出現,收獲病毒液.加人聚凝胺感染H446細胞,並篩選出含TK基因的H446細胞....
HSV-tk gene therapy for human renal cell carcinoma in nude mice. Kalevi Juhani Pulkkanen,Jyrki J Parkkinen,Johanna M Laukkanen,Mikko I Kettunen,Kristiina Tyynela,Risto A Kauppinen,Martti Y Ala-Opas,Seppo Yla-Herttuala. Cancer Gene Therapy . 2001...
transducted into human bladder cancer cell T24. HSV-TK gene transfection with GCV can effectively inhibit growth of human bladder cancer cell T24. The results suggest that therapy of HSV-TK gene will be a potential possibility of treatment of human bladder cancer. Key words:HSV-TK gene;gene ...
The plasmid pACCMVTK that expresses simple virus thymidine kinase (HSV-TK) gene was constructed,and recombinant adnovirus AdCMVTK was gained. Glioblastoma cells TJ905 were infected by AdCMVTK.The effect of HSV-TK/GCV system to growth of glioma cell was observed.The results showed HSV-TK/GCV...
关键词:HSV 1 -TK 基因;双歧杆菌;原核表达;基因治疗中图分类号:Q786 文献标识码:A 文章编号:1004-311X(2008)06-0011-04Construction of Prokaryotic Expression Plasmid Included HSV 1 -TKGene and Detection of Expressed ProteinWANG Li -qun1, ZHANG Ming-jiang2, MENG Xiang-chen1*(1.Key Laboratory ...
HSV-TKHerpes Simplex Virus Thymidine Kinase Gene Copyright 1988-2018AcronymFinder.com, All rights reserved. Suggest new definition Want to thank TFD for its existence?Tell a friend about us, add a link to this page, or visitthe webmaster's page for free fun content. ...
Preparation of HER2-targeted nano-ultrasound contrast agent carrying HSV-TK gene and with nuclear localization effect ZHANG Yue1，ZHOU Houren，LUO Yucen，XIN Ying，LIU Xiaoqi，LIU Zhijun 1Shengjing Hospital of China Medical University，Shenyang110004，China Abstract：Objective To prepare a human ...
获得重组HSV病毒的经典方法是选择tk基因作为同源臂,将外源基因表达盒插入到HSV1基因组中,经过筛选获得重组病毒。 我们实验室建立了一套基于粘粒系统的HSV1重组HSV1病毒方法,直接用分子克隆的方法在HSV1基因组中插入外源基因表达单位。本实验室曾将4.5kb的lacZ基因表达盒(CMV-lacZ-polyA...
有HSV-tk基因的C6/tk细胞凋亡,以上结果为胶质瘤基因治疗的研究提供依据。 关键词 胶质瘤 凋亡 基因治疗 环丙氧苷 中图号 R73-35 + 4 GanciclovirInducesApoptosisofRatC6GliomaCellTransducedwithHSV-tkGene △ MaWenbin # ChenSongshen * RenZuyuan WangWeijun ...
单纯疱疹病毒胸苷激酶(HSV-tk)基因是肿瘤基因治疗研究中常用的自杀基因。其作用原理,是因为某些特定的酶蛋白基因如HSV … baike.baidu.com|基于140个网页 2. 单纯疱疹病毒胸苷激酶基因 单纯疱疹病毒胸苷激酶基因(HSV-TK)/丙氧鸟苷(GCV)系统4) Deoxyguanosine 脱氧鸟苷 更多例句>> 5) HSV-TK/GCV 单纯疱疹 … ...