HSV-tk gene therapy for human renal cell carcinoma in nude mice. Kalevi Juhani Pulkkanen,Jyrki J Parkkinen,Johanna M Laukkanen,Mikko I Kettunen,Kristiina Tyynela,Risto A Kauppinen,Martti Y Ala-Opas,Seppo Yla-Herttuala. Cancer Gene Therapy . 2001...
transducted into human bladder cancer cell T24. HSV-TK gene transfection with GCV can effectively inhibit growth of human bladder cancer cell T24. The results suggest that therapy of HSV-TK gene will be a potential possibility of treatment of human bladder cancer. Key words:HSV-TK gene;gene ...
单纯疱疹病毒胸苷激酶基因(HSV-TK)3) HXV-TK gene 单纯疱疹病毒胸苷激酶(HXV-TK)基因4) herpes simplex virus thymidine kinase/gancclovir (HSV-TK/GCV) 单纯疱疹病毒胸苷激酶基因(HSV-TK)/丙氧鸟苷(GCV)系统5) Herpes simplex virus thymidine kinase gene 单纯疱疹病毒胸苷激酶基因 例句>> 6...
单纯疱疹病毒胸苷激酶(HSV-tk)基因是肿瘤基因治疗研究中常用的自杀基因。其作用原理,是因为某些特定的酶蛋白基因如HSV … baike.baidu.com|基于140个网页 2. 单纯疱疹病毒胸苷激酶基因 单纯疱疹病毒胸苷激酶基因(HSV-TK)/丙氧鸟苷(GCV)系统4) Deoxyguanosine 脱氧鸟苷 更多例句>> 5) HSV-TK/GCV 单纯疱疹 … ...
HUVEC+HUVEC/tk混合细胞的存活率,随HUVEC/tk细胞比例的增加而降低,说明腺病毒介导的KDR启动子-单纯疱疹病毒胸苷激酶/丙氧鸟苷系统不但能杀伤tk+细胞,也能杀伤未导入tk的细胞,存在明显的旁观者效应。 2. Methods: HSV-TK gene was transfected into PC-3m cells through retrovirus vector, reverse transcription-pol...
Preparation of HER2-targeted nano-ultrasound contrast agent carrying HSV-TK gene and with nuclear localization effect ZHANG Yue1，ZHOU Houren，LUO Yucen，XIN Ying，LIU Xiaoqi，LIU Zhijun 1Shengjing Hospital of China Medical University，Shenyang110004，China Abstract：Objective To prepare a human ...
Effect of Regulation of HSV-tk Gene Expression and Tumor Killed Activity with a Single Tetracycline-regulatable Plasmid Vector on HeLa Cells HeLa细胞tk基因载体系统细胞活性四环素调控pcDNA3.1To construct a single tetracycline-regulatable plasmid vector based on the double tetracycline-regulatable plasmid .....
HSV-TK基因靶向治疗与放射治疗联合应用的研究进 展 作者作者单位 谭祥四川,泸州医学院附属医院肿瘤科 1HSV-TK/GCV的作用机制 HSV-TK发挥对肿瘤细胞的杀伤作用主要体现在直接抑制肿瘤 生长和旁观者效应两个方面。 1.1直接抑制肿瘤生长 HSV-TK基因能将无毒性的核苷酸类似物转化成有毒性的物质, ...
The efficacy of expression of the herpes simplex virus thymidine kinase (HSV-tk) gene under the transcriptional control of the liver-specific albumin gene promoter, followed by ganciclovir treatment, was investigated both in vitro and in vivo. Murine and rat hepatocellular carcinoma (HCC) cells infe...
获得重组HSV病毒的经典方法是选择tk基因作为同源臂,将外源基因表达盒插入到HSV1基因组中,经过筛选获得重组病毒。 我们实验室建立了一套基于粘粒系统的HSV1重组HSV1病毒方法,直接用分子克隆的方法在HSV1基因组中插入外源基因表达单位。本实验室曾将4.5kb的lacZ基因表达盒(CMV-lacZ-polyA...