使用重组单纯疱疹病毒2(HSV-2)疫苗载体的免疫的被动转移.pdf,使用重组单纯疱疹病毒2(HSV‑2)疫苗载体、其病毒颗粒、包含其的组合物和疫苗的免疫被动转移的方法。(19)中华人民共和国国家知识产权局 (12)发明专利申请 (10)申请公布号 CN 113727728 A (43)申请公布日 2021.11
用强毒HSV-2攻击后,用ΔgD-/+按皮下-阴道内方式免疫的小鼠显示阴道中抗HSV-2gBT-ICD8+T细胞(C)和CD4+T细胞(D)的数目增加。加工阴道组织以提取T细胞并用抗体染色以便流式细胞分析。用(CountBrightTM,Lifetechnologies)进行细胞计数。(*p<0.05;**p<0.01)。发明详述提供一种分离的重组单纯疱疹病毒-2(HSV-2),...
According to the manufacturer’s protocol, VUs represent the 10-times quotient of the samples’ optical density (OD) and the OD of a kit-provided cut-off control. VU > 11 is considered positive, while VU < 9 is determined negative. VU values between 9 and 11 are considered equivocal/...
Thus, HSV1 and HSV2 preferentially replicate and establish latency in different populations of sensory neurons (Table 1) [6,7,9,10]. However, these preferred sites of latency only account for about half of the latent sites and it is not clear whether the viruses reactivate from these ...
PCR parameters consisted of 10 min FastStart Taq activation at 95 ◦C, followed by 40 cycles of 95 ◦C for 20 s, and 60 ◦C for 1 min. Specificity was verified by melting curve analysis. GAPDH signal was normalized to the 18S rRNA signal using the 2(−∆∆Ct) method [36,...
6 of 16 a 3T3 3T6 1×106 1×105 NS * ** 1×104 1×103 1×102 1×101 24 48 Hours 72 c NS b 3T3 48)hour)MOI)1 3T6 48)hour)MOI)1 d 2.0×106 3T6 MOI 10 1.5×106 3T6 MOI 5 3T6 MOI 1 1.0×106 * 5.0×105 0.0 0 * 9 21 33 45 57 69 Hours FFiigguurree 22.. ...