For instance, the blockade of free IgE by omalizumab (anti-IgE monoclonal antibody) showed some clinical benefit in AD patients with poor response to traditional therapy (Wang et al. 2016). In addition, clinical evidence suggests that targeting IgE antibodies by immunoadsorption seems to be an ...
The Omicron BA.1 variant of SARS-CoV-2 circumvents the neutralizing power of convalescent sera and monoclonal antibodies targeting earlier strains. The immune system's evasion is largely attributable to mutations within the BA.1 receptor binding domain (RBD), the key antigenic target of the SARS...
Currently used treatment modalities for SARS-CoV-2 infection include repurposed small molecules such as remdesivir (Beigel et al., 2020), immunomodulatory agents such as corticosteroids (Prescott and Rice, 2020), and virus-specific monoclonal antibodies (Zhou et al., 2020b). However, most of ...
To verify the glutathionylation status of Hsp90 in cells, we performed an immunoprecipitation assay with specific antibodies and detected glutathionylation signals using a glutathione-specific antibody. As shown in Fig. 1A, Fig. S1 and Fig. S7A, the glutathionylation signals were strongly ...
Rabbit monoclonal GR XP (DH82) antibody (Cell Signaling Technology, 1:1000, cat. no. 3660) was raised against a peptide corresponding to residues surrounding Leu378 of the human GR protein and recognizes the GRα-A (94 kDa), GRα-B (91 kDa), GRα-C (82 kDa), and GRα-D (54 ...
the ImageJ software (U. S. National Institutes of Health, Bethesda, MD, USA;http://rsb.info.nih.gov/ij) and normalized to α-tubulin values using mouse monoclonal α-tubulin (Sigma-Aldrich) with polyclonal HRP-conjugated sheep anti-mouse IgG antibody (GE Healthcare, Fairfield, CT, USA)....
If tumour-secreted exosomes carry eHsp90α on their external surface, we anticipated that an anti-eHsp90α neutralizing monoclonal antibody would block the communication from tumour-secreted exosomes to stromal cells, since antibodies do not penetrate the lipid membrane of exosomes. We tested the mo...
PPARg was immunoprecipitated by a monoclonal anti-PPARg antibody. Pellets were washed five times with lysis buffer and analyzed by SDS-PAGE and immunoblotting with anti-Hsp90 and anti-PPARg antibodies. Statistical analysis. Data were compared by two-tailed unpaired t-test. Variables were expressed ...
Prior therapy had to be completed >4 weeks (>6 weeks for nitrosoureas) previously and monoclonal antibody therapy >6 weeks prior to entry. A stable dose of bisphosphonate treatment for >3 months prior to entry was permitted.Patients had to have adequate haematological (absolute neutrophil count ...
Assays for HSP90 and Inhibitors英文文献.pdf,HSP90 Inhibition Assays 149 14 Assays for HSP90 and Inhibitors Wynne Aherne, Alison Maloney, Chris Prodromou, Martin G. Rowlands, Anthea Hardcastle, Katherine Boxall, Paul Clarke, Michael I. Walton, Laurence Pe