The relationship between drug concentration (pharmacokinetics) and its biological effects (what the drug does to the body). Trastuzumab A humanized monoclonal antibody that binds HER2 on tumour cells and prevents uncontrolled proliferation caused by aberrant HER2 signalling. RECIST A set of published rul...
The papain digestion of monoclonal antibodies is a frequent method of producing the antigen-binding fragments (Fabs) necessary for research. However, the dynamic between papain and antibodies at the interaction site is still unclear. At liquid-solid interfaces, we developed ordered porous layer ...
Myc-tagged proteins were detected with mouse anti-myc monoclonal antibody65 (1:100; 4A6 Millipore, cat- alog number 05-724) and Hsp82p was detected with anti-Hsp90 antibody (1:1000; Anti-Hsp90, Clone K41220A, Stressmarq Biosciences Inc., Victoria, BC, Canada; catalog number SMC-135),...
HSP90 is moderately overexpressed in drug-resistant relative to parental cells, but HSP90 inhibition with onalespib (0.5 µM) selectively induced apoptosis in resistant cells only, marked by dramatic increase of cleaved poly(ADP-ribose) polymerase (Cl PARP) and caspase 7 (Cl Cas7) at 24...
Monoclonal antibodyTumor-associated antigensStemnessBackground: Identification of promising targeted antigens that exhibited cancer-specific expression is a crucial step in the development of novel antibody-targeted therapies. We here aimed to investigate the anti-tumor activity of a novel monoclonal ...
抗体名称Anti-Hsp90 alpha/HSP90AA1 Antibody (Clone#6B5) 指标别名Heat shock 86kDa;HSP 86;HSP86;Hsp89;HSP89A;HSP90;HSP90A;HSP90AA1;HSP90N;HSPC1;HSPCA;HSPCAL1;HSPCAL4;HSPN;LAP2 克隆性Monoclonal 检验物种human,monkey,mouse,rat 应用范围WB,IHC,ICC/IF,FCM ...
the membranes were incubated overnight at +4 °C with a rabbit monoclonal NLRP3 antibody (1:1000, Abcam, Cambridge, UK). After washing three times for 5 min, the membranes were incubated for 2 h at RT with horseradish peroxidase-conjugated anti-rabbit IgG antibody (1:5000, Life Te...
Next, we utilized a monoclonal antibody that specifically bound to Conclusion In conclusion, we synthesized a series of coumarin pyrazoline compounds HCP1-HCP6 that might be HSP90 inhibitors according to former studies. The infusive observations revealed that HCP1-HCP6 could inhibit the activity of...
Monoclonal poliovirus antibodies were generously provided by Dr. Morag Ferguson at NIBSC, Blanche Lane, South Mimms, Potters Bar, Hertfordshire. Antibody neutralizations and competition assays For selection of antibody escape mutants, ~105 (mAb427) or 106–107 (mAb423) plaque forming units (PFU) ...
The antibody recognizing the N-terminus of p100 (anti-p100N) was described previously [16]. The monoclonal antibodies for human CD40 and Myc were prepared from hybridomas G28-5 and 9E10, respectively [12]. 5-aminoimidazole-4-carboxamide 1-b- D-ribofuranoside (AICAR) and MG132 were from ...