Enzyme conjugated antibodies offer the most flexibility in detection and documentation methods for Western blotting because of the variety of substrates available. The simplest detection/documentation system is to use chromogenic substrates. While not as sensitive as other substrates, the chromogenic substrat...
免疫印迹(Western Blot):通过将抗体标记上HRP,再利用链霉亲和素-HRP复合物来检测目标蛋白。 ELISA(Enzyme-Linked Immunosorbent Assay):通过将抗原或抗体标记上生物素,然后利用链霉亲和素-HRP复合物进行检测。 核酸探针检测:通过将核酸探针标记上生物素,再利用链霉亲和素-HRP复合物进行信号放大和检测。
英文名称:Uracile Nicking Enzyme 规格:50U 本产品在尿嘧啶位置产生一个单核苷酸缺口,它是尿嘧啶DNA糖基化酶(UDG)和DNA糖基化酶-裂解酶 Endo Ⅷ的混合物。UDG催化尿嘧啶碱基的切割,形成一个脱碱基(脱嘧啶)位点,但保持磷酸二酯骨架结构完整。Endo Ⅷ的裂解酶活力使脱碱基位点 3′和5′端的磷酸二酯键断裂,释...
Horseradish Peroxidase (HRP)is an oxidative enzyme with numerous applications in biochemistry due to its stability and the variety of its substrates. Through the use of different chemiluminescent and colorimetric substrates, HRP can be used to detect antigens in Western blot, quantify targets in ELISA...
链霉亲和素是一种来自streptomyces avidinii菌的非糖基化蛋白。链亲和素同源四聚体对生物素具有特别高的非共价结合亲和力。当与酶(如辣根过氧化物酶或碱性磷酸酶)形成偶联物并与比色或发光底物发育系统结合时,链霉亲和素与生物素化抗体在许多应用中得到广泛应用,包括Western blot、ELISA、ELISPOT、免疫细胞化学和免疫...
FemtoMax™ is designed for both western blotting and enzyme immunoassay (EIA) use. FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras. Blots can be repeatedly exposed to ...
Horseradish Peroxidase (HRP) is an oxidative enzyme with numerous applications in biochemistry due to its stability and the variety of its substrates. Through the use of different chemiluminescent and colorimetric substrates, HRP can be used to detect antigens in Western blot, quantify targets in ...
背景 Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey...
Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is...
WB, Western blot; ELISA, Enzyme-linked Immunosorbent Assay; IHC, Immunohistochemistry. 本辣根过氧化物酶标记山羊抗兔IgG(H+L)(HRP-labeled Goat Anti-Rabbit IgG(H+L))为进口分装,用于Western、ELISA和IHC等的二抗。HRP,即horseradish peroxidase,中文名为辣根过氧化物酶,可以在Western检测时催化ECL类试剂(如...