Enzyme conjugated antibodies offer the most flexibility in detection and documentation methods for Western blotting because of the variety of substrates available. The simplest detection/documentation system is to use chromogenic substrates. While not as sensitive as other substrates, the chromogenic substrat...
Horseradish Peroxidase (HRP)is an oxidative enzyme with numerous applications in biochemistry due to its stability and the variety of its substrates. Through the use of different chemiluminescent and colorimetric substrates, HRP can be used to detect antigens in Western blot, quantify targets in ELISA...
免疫印迹(Western Blot):通过将抗体标记上HRP,再利用链霉亲和素-HRP复合物来检测目标蛋白。 ELISA(Enzyme-Linked Immunosorbent Assay):通过将抗原或抗体标记上生物素,然后利用链霉亲和素-HRP复合物进行检测。 核酸探针检测:通过将核酸探针标记上生物素,再利用链霉亲和素-HRP复合物进行信号放大和检测。 这种结合不仅提...
FemtoMax™ is designed for both western blotting and enzyme immunoassay (EIA) use. FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras. Blots can be repeatedly exposed to ...
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HRP (horseradish peroxidase) is an enzyme frequently used as an indicator in biochemical assays. It is used to amplify signals and enhance a target’s detectability. HRP acts on various chromogenic substrates to produce many different colors and acts on chemiluminescent substrates to pr...
英文名称:Uracile Nicking Enzyme 规格:50U 本产品在尿嘧啶位置产生一个单核苷酸缺口,它是尿嘧啶DNA糖基化酶(UDG)和DNA糖基化酶-裂解酶 Endo Ⅷ的混合物。UDG催化尿嘧啶碱基的切割,形成一个脱碱基(脱嘧啶)位点,但保持磷酸二酯骨架结构完整。Endo Ⅷ的裂解酶活力使脱碱基位点 3′和5′端的磷酸二酯键断裂,...
Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is...
This Streptavidin-HRP enzyme conjugate is intended for immunoassays such as ELISA, ELISpot, and Western blot.
Horseradish Peroxidase (HRP) is an oxidative enzyme with numerous applications in biochemistry due to its stability and the variety of its substrates. Through the use of different chemiluminescent and colorimetric substrates, HRP can be used to detect antigens in Western blot, quantify targets in ...