HRP酶标二抗(Horseradish Peroxidase-conjugated Secondary Antibody)是指通过与辣根过氧化物酶(HRP)偶联的二级抗体。这种二抗在生物医学研究中广泛应用,尤其在免疫检测技术如酶联免疫吸附试验(ELISA)、蛋白质印迹(Western Blot)和免疫组织化学(IHC)等中,具有不可替代的作用。 一、HRP酶标二抗的工作原理 HRP酶标二抗的工作...
这里的“HRP”是指辣根过氧化物酶,它是一种广泛应用的酶标记物,能够在多种免疫检测中催化底物产生可检测的信号。在Western Blot、ELISA(酶联免疫吸附试验)和IHC(免疫组织化学)等实验中,二抗金标准Jackson的HRP二抗常用于检测目标蛋白或抗体。 具体来说,HRP二抗能够与一抗(Primary Antibody)结合,一抗则特异性地识别...
SA00001-18 (HRP-conjugated Protein A from S.aureus) as secondary antibody.Enlarge Image Product Information Concentration 0.2 mg/mL Applications WB, Dot blot Host Species Species Reactivity FC Fragment Physical State Liquid Suggested Dilution Range 1:4000-1:10000 for Western Blotting with ...
Western blot was performed using Mouse anti-Human IgE Secondary Antibody, HRP (Product # MA5-16454) and a 73 kDa band corresponding to Human IgE Heavy Chain was observed in IgE but not in IgG1, IgG2, IgG3, IgG4, IgA, IgM, Mouse IgG, Mouse IgM and Rabbit IgG (Panel I). Purified...
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins...read more • ...
Various lysates were subjected to SDS PAGE followed by western blot with Biotin-60293 (OPTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SA00001-0 (HRP-conjugated Streptavidin) as secondary antibody, at dilution of 1:3000 (lane 1 and 3) or 1:6000 (lane 2...
Rabbit IgG (H+L) Secondary Antibody (31460) in IHC (P) Immunohistochemistry was performed on human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave. Following antigen retrieval...
Rabbit IgG (H+L) Secondary Antibody (31460) in IHC (P) Immunohistochemistry was performed on human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes using a microwave. Following antigen retrieval...
Secondary Antibody 抗原 Full length native Goat IgG (purified). 偶联物 HRP 查看其它偶联染料 形式 Liquid 浓度 2 mg/mL 纯化类型 Antigen affinity chromatography 保存液 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA 内含物 0.01% gentamicin sulfate 保存条件 ...
产品编号31471已成功用于Western blot,ICC,IHC和IP应用。 产品编号31471与大鼠IgG的重链和大多数大鼠免疫球蛋白共有的轻链发生反应,但不与非免疫球蛋白血清蛋白发生反应。但是,该抗体可能与其他物种的免疫球蛋白发生交叉反应。 将产品存放在4°C直至打开。为了延长该产品的保质期,请添加等体积的甘油以使甘油的最终浓度...