A thorough study of gene expression and changes in a group of DNA DSB repair genes in HPV-negative and HPV-positive HNC cancers also shows differences, suggesting that HPV affects the DNA repair processes in th
PCR: Polymerase chain reaction QPCR: Quantitative real-time polymerase chain reaction LAMP: Loop mediated isothermal amplification RCA: Rolling circle amplification RAA: Recombinase-aidamplification crRNA: CRISPR RNA gRNA: Graduate RNA ssDNA: Single-stranded DNA ssRNA: Single-stranded RNA PAM...
PCR: Polymerase chain reaction ASUGI: Azienda Sanitaria Universitaria Giuliano Isontina HR-HPV: High-risk human papillomavirus OR: Odd ratios CIN: Cervical intraepithelial neoplasia CE-IVD: CE-marked in-vitro diagnostics CI: Confidence interval WHO: World Health Organization LSIL: Low-grad...
PCR: Polymerase chain reaction QPCR: Quantitative real-time polymerase chain reaction LAMP: Loop mediated isothermal amplification RCA: Rolling circle amplification RAA: Recombinase-aidamplification crRNA: CRISPR RNA gRNA: Graduate RNA ssDNA: Single-stranded DNA ssRNA: Single-stranded RNA PAM...
Detection of HPV DNA and other STI DNA was extracted from CVL pellets using an automated MagNA pure instrument (Roche Diagnostics, Indianapolis, IN, USA) according to the manufacturer’s instructions. HPV DNA was amplified by PCR using the Roche Linear Array® HPV Genotyping kit (Roche Diagnos...
The mean (SD) lead time from positive test to recurrence date was 122 (169.8) days. Meaning The results of this cohort study suggest that posttreatment presence of HPV DNA in plasma and salivary rinses is associated with recurrence and potentially offers an opportunity for earlier detection of ...
(ratio ≥ 4,n = 127). HPV16-positive patients consistently had a higher HPV copy number (n = 95/155, 61%) (p < 0.0001) as compared to patients with other HPV subtypes (n = 32/117, 27%) (Table3). Samples with 2J type insertions displayed a low HPV copy...
Thus, the combination of colposcopy and hrHPV PCR testing in a hrHPV-positive patient collective of no- or low-grade cytologic abnormali- ties achieves similar diagnostic accuracy as colposcopy alone in a patient collective of patients with history of abnormal PAP smears. This result is quite ...
The present invention also relates to an HPV E6 or E7 protein which is encoded by the above described DNA sequences. The HPV E6 or E7 protein is provided as isolated, purified material, and therefore free of other proteins. Such HPV proteins are, preferably, expressed in human cells and, ...
Typically, amplification reagents along with other reaction components are placed and contained in a reaction vessel (test tube, microwell, etc.).With PCR, it is possible to amplify a single copy of a specific target sequence in genomic DNA to a level detectable by several different methodologies...