7. Normal-Phase Chromatography 8. System Volume, Dead Volume, Dwell Volume 9. Transfer of Gradient Methods 10. Clogged System 11. Column Plate-Count 12. Column Backpressure 13. Peak Area Fluctuations 14. Ghost Peaks 15. Dependence of Retention Times on pH 16. Column Equilibration 17. Column ...
These will be combined to create a series of calibration solutions that will allow us to determine which peak is which, and what the relationship is between the peak area and the concentration of the standard.这些都将结合创建一系列的校准解决方案,将使我们能够确定峰,峰面积和浓度的标准之间的关系...
Even if air bubbles do not appear, changes in the dissolved gas amount may cause baseline fluctuations. To avoid such troubles, it is important to remove (degas) the dissolved gas in the eluent. There are two methods for degassing the eluent: one is to degas the eluent in advance (...
The peak height (h) is the vertical distance between a peak's apex and the baseline, and the peak area (A) colored in light blue is the area enclosed by the peak and baseline. These results will be used for the qualitative and quantitative analysis of a sample's components. ...
These will be combinedto create a series of calibration solutions that will allow us to determine which peak is which, and what the relationship is between the peak area and the concentration of the standard.这些都将结合创建一系列的校准解决方案,将使我们能够确定峰,峰面积和浓度的标准之间的关系是...
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•ThereareawidevarietyofstationaryphasesavailableforHPLC.Inthislabwewilluseanormalphase(Silicagel),althoughreversephase(silicagelinwhicha18carbonhydrocarboniscovalentlyboundtothesurfaceofthesilica)columnsarecurrentlyoneofthemostcommonlyusedHPLCstationaryphases.HistoryofHPLC–M.S.Tswett1903•Calciumcarbonatecolumnsto...
Sources of Peak Tailing 7. Normal-Phase Chromatography 8. System Volume, Dead Volume, Dwell Volume 9. Transfer of Gradient Methods 10. Clogged System 11. Column Plate-Count 12. Column Backpressure 13. Peak Area Fluctuations 14. Ghost Peaks 15. Dependence of Retention Times on pH 16. Column ...
The reference beam and the reference photodiode are used to compensate for light fluctuations from the lamp. For optimum sensitivity, 89 8 Diode array detectors the UV detector can be programmed for each peak within a chromatographic run, which changes the wavelength automatically. The variable ...
Diluted QCs were prepared to distinguish between informative and uninformative signals; for each metabolic feature the correlation factor between the peak area and the dilution factor was calculated. Only the features which showed a coefficient > 0.8 were maintained in the dataset while the others...