5) Calculation Determine the peak areas of the Maglolol on the sample chromatogram and compare to the corresponding peaks in the chromatogram for the standard solution. Calculate the weight percent.
for about 10 minutes and transfer to screw-cap vialsand can be stored in the refrigeration.5) Calculation Determine the peak areas of the Maglolol on the sample chromatogram and compare to the corresponding peaks in the chromatogram for the standard solution. Calculate the weight percent.
WhenqualifyingHPLCequipment,itshouldbenotedthatitisacceptabletocheckat LevelIIIandIVseveralofthementionedparametersatthesametimeinacombinedtestprocedure.“overall”systemperformancetestgivinginformationonpeakarea precision,retentiontimeprecision,gradientreproducibility,etc). 在进行HPLC仪器确认时,应注意也能够对第三级和第...
峰面积重复性:对照液(b)色谱图中所有峰的峰面积相对标准偏差应≤1.0 % Carry-over: Thepercentage of the peak area corresponding to propylparabene in the blankinjection 1 does not exceed 0.5% of 10 times the peak area of thepropylparabene peak in the chromatogram obtained with the reference ...
[Results] The results showed that when the content of limonene were among 0.21 -1.05 mg/ml,good linear dependence of peak area on concentration of limonene and the correlation coefficient R2 was 0.995,and the average recovery was 98.0% ,RSD was 2.6% ; after the calculation,the content of ...
欧盟OMCL仪器确认附件1-HPLC确认.pdf,BEIJING GELEG SCI.-TECH.CO.LTD OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT PA/PH/OMCL (11) 04 QUALIFICATION OF EQUIPMENT 仪器确认 ANNEX 1: QUALIFICATION OF HPLC EQUIPMENT 附件1:HPLC 仪器的确认 Ful
学WHENQUALIFYINGHPLCEQUIPMENT,ITSHOULDBENOTEDTHATITISACCEPTABLETOCHECKATLEVELIIIANDIVSEVERALOFTHEMENTIONEDPARAMETERSATTHESAMETIMEINACOMBINEDTESTPROCEDURE(E.G.“OVERALL”SYSTEMPERFORMANCETESTGIVINGINFORMATIONONPEAKAREAPRECISION,RETENTIONTIMEPRECISION,GRADIENTREPRODUCIBILITY,ETC).在仪行HPLC仪器仪仪,仪注意也可以仪第三仪...
light.5) Calculation Under the above chromatographic conditions, inject the sample for determination after the instrument stabilizes and the baseline is stable. The retention time of Fisetinis about 10 minutes. Calculate the content using the external standard method based on the area of each peak.
(1.TCMResearchCenter,ZhejiangAcademyofTCM,Hangzhou310007,China;2.CollegeofPharmaceuticalScience,ZhejiangUniversityofTCM,Hangzhou310053,China)ABSTRACTOBJECTIVE:ToevaluatethequalityofAtractylodesmacrocephalaefromthetypeofchemicalcomponentandpeakareaofcharacteristiccomponent.METHoDS:TheHPLCfingerprintofethanolextractfromA....
Areafound Figure3.5.Multi-pointcalibration. ImprovedMethodSensitivity.Inthisapproach,onlytheresponsesofindividual relatedsubstancesareusedinthecalculation.Sincetheareaofdrugsubstance peakinthesampleinjectionsisnotnecessaryforthecalculation,highsample concentrationscanbeusedwithoutworryingabouttheoff-scaleresponseofthe ...