The separation was carried out by using phenomenex C18 column (15cm×4.6mm id, 5μm particle size) and UV detection at 239nm. The ranges of the independent variables used for the optimization were MeCN: 33–38%, buffer conc.: 10–20mM and flow rate: 1–2ml/min. The influence of ...
Methods: The Phenomenex C18column( 250 mm × 4. 6 mm,5 μm) and the diode array detector( DAD) were used for separation and detection,respectively. The separation process was carried out using a gradient elution( 0- 15 min,2 %B→4 % B; 15- 50 min,4 % B →16 % B; 50- 57 ...
HPLC analyses of methamphetamine and pseudoephedrine extracted from urine samples were performed using a C18 chromatography column (250 × 4.6 mm, 5 μm, pre-column, Berlin) by applying a mobile phase composed of acetonitrile-phosphate buffer (pH = 2.8, 10 mM), (15:85, v/v). The...
Some of the most popular column families are: ACQUITY UPLC Columns: ACQUITY UPLC columns are specifically designed for ultra-performance liquid chromatography systems, such as the ACQUITY UPLC and ACQUITY Arc systems. These columns utilize smaller particle sizes, typically less than 2 μm, which ...
of high resolution chromatographic peaks, as is evident from the many injectors and detectors optimized and available for open tubular GC. A particle packed column is comprised of a Nano litre enrichment column and a micron or sub-micron separation column packed with suitable grade of C18. ...
100-C18 column(25cm×4.0mm×5μm) at 35℃ with a mobile phase of 98% methanol. The flow rate was 1.0ml/min. a-Tocopherol was detectedat 280nm. The range of linearity was from 0.2mg/L to 100mg/L (r=0.9997). The lower limit of detection of α-Tocopherol was0.2mg/L, The ...
Waters Atlantis T3 RP-C18 colume(4.6mm×150mm,5μm),Waters Atlantis T3 Security Guard(4.6mm×1.5mm,5μm),a mobile phase of 0.02mol/L KH2PO4(pH2.7),isocratic elution with a flow rate of 0.8 mL/min,a column temperature of 40℃ and detection wavelengths at 210nm and 243nm.This metho...
as is apparent from the numerous injectors and sensors optimized and available for open tubular GC. A flyspeck-packed column is comprised of a Nano liter enrichment column and a micron or sub-micron separation column packed with a suitable grade of C18. The HPLC- Chip is made from a biocomp...
[Objective] To establish the HPLC method for determination of glabridin in G.glabra L.[Methods] The TIANHE C18 column(250 mm×4.6 mm,5 μm) was used.The mobile phase was acetonitrile-water-glacial acetic acid(55:44:1).The detection wavelength was 282 nm.The column temperature was 30 ℃...
The chromatographic separation was performed on a Phenomenex Luna C18 column (150 X 4.0 mm, 5m) in isocratic mode using acetonitrile and 0.02M potassium phosphate buffer (50 + 50, v/v, pH 5.0 adjusted with potassium hydroxide) as the mobile phase at a flow rate of 1.0 ml/min. The ...