The quantification was carried out using C18 column and mobile phase consisting of acetonitrile: 20mM phosphate buffer at pH 3 (25:75v/v), at flow rate of 1 ml/min. The separation was performed at ambient temperature. Eluents were monitored by UV detector set at 275 nm. The method was ...
22.20 7.20 1.60 XSelect HSS T3 2.23 1.19 6.50 3.15 1.60 XTerra RP18 1.00 1.20 1.70 1.10 2.30 XTerra MS C18 1.50 1.10 3.30 1.30 2.20 * For use in C18 column brand name selection when referencing USP monographs which specify the characteristics/performance of the C18 column used in the method...
5 μm), Thermo scientific Hypersil C8 column (150 mm × 4.6 mm i.d., 5-µm) and Thermo scientific Hypersil C18 column (150 mm × 4.6 mm i.d.,
of high resolution chromatographic peaks, as is evident from the many injectors and detectors optimized and available for open tubular GC. A particle packed column is comprised of a Nano litre enrichment column and a micron or sub-micron separation column packed with suitable grade of C18. ...
Separation was accomplished on Eclipse plus C18 (100 mm × 4.6 mm × 3.5 μm) column with controlled pore size of 95A using a mixture of 27% (v/v) isopropanol and 73% (v/v) 20 mM potassium dihydrogen phosphate buffer (pH adjusted to 3.0) as mobile phase at a flow rate ...
Optimum separation was achieved in less than 10 min using an X-Bridge C18 column (150 mm × 4.6 mm i.d., 3.5 μm particle size); elution was accomplished via the application of a dual-mode solvent and flow rate gradient system. This elution system enables the separation of nine ...
When column chemistries are different, try a modified method development approach Proposed Strategy 1: —Understand general column selectivity differences —Follow chemistry and system scaling protocols —Generic gradients : 10 to 90 % organic solvent —Run a short gradient (3 min) and a ...
The com- parison method was an isocratic HPLC method using C18 column with mobile phase consisting of methanol: sodium phosphate dibasic anhydrous buffer (65:35 v/v) at pH of buffer 7.0 ± 0.02. It was found that the calculated values for Student's t-test and the variance ratio F...
The components of each sample were separated and detected by an HPLC system using a LPG-3400RS quaternary pump, WPS-3000TRS autosampler (maintained at 20°C), TCC-3000RS column oven (maintained at 40°C), Accucore C18 column (150 × 3.0 mm, 2.6 μm particle size), FLD-3100 ...
For further isolation, the hapten was separated by a preparative liquid chromatography using a C18 (10-μm) column (25-mm i.d. × 600-mm length; lisui II) with a UV detector (Lisui, Suzhou Lisui Technology Co. Ltd., Suzhou, China). The gradient used was 100% water to 100% ...