Guinchard. Study and optimization of column efficiency in HPLC:comparison of two methods for separating ten benzodiazepines. J. Liq. Chromatogr. 17: 1443-59 (1994).Guillaume, Y.; Guinchard, C. Study and optimization of column effi- ciency in HPLC: comparison of two methods for separating ...
Although the column is the heart of the HPLC instrument and essential to its success, until now no single book has focused on the theory and practice of column technology. 'HPLC Columns' provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and ...
From the pareto charts results, three factors which have the greatest significance were selected for further optimization including ratio of organic modifier, pH of phosphate buffer, and column temperature. Other factors were set at their values that result in better method greenness and chromatographic...
• HPLC&GC development method&optimization • Contract laboratory • Buffer Mfg • Custom SPE column&plate Mfg 21 Historical development of Interchim’s chromatography technologies: 2007 StrategyTM 1.7µm and 2.2µm Stationary phase ultra fast analysis&high throughput Modulo-cart™...
urine and other body fluids. HPLC consists of using a liquid mobile phase to pass under high pressure a mixture of analytes extracted from the sample through a column containing the stationary phase.Analyte separationis based on differences in interaction with both the mobile phase and the stationa...
· Imad Ahmad of Merck, in-silico multifactor modeling for optimization of 2D-LC. Merck公司的Imad Ahmad,用于优化2D-LC的硅酮多因素模型 · Bingchuan Wei of Genentech, Bridging structure and function of biotherapeutics by emerging chromatographic technologies. ...
Agilent A-Line Quick Connect Fittings The fast, easy way to more predictable results Poorly connected flow path fittings are one of the top reasons for peak tailing, peak broadening, split peaks, and carryover. That's because dead volume or micro-leakage can cause extra-column volume, which ...
Optimization of Chromatographic Conditions Chromatography was performed using an XTerra MS C18 (150.0 × 3.0 mm i.d., 5 μm particle size) column, while chromatographic conditions were optimized to separate quercetin–aluminium complex from the excess of quercetin and the cream matrix excipients. Meth...
Derringer’s desirability function as as a multi-criteria approach was applied for getting the optimum chromatographic analysis conditions. Efficient analysis of BET and CAR was achieved at column temperatures of 26.00 and 27.50 °C, respectively using acetonitrile and phosphate buffer (pH 4.55) 70:...
(maintained at 20°C), TCC-3000RS column oven (maintained at 40°C), Accucore C18 column (150 × 3.0 mm, 2.6 μm particle size), FLD-3100 fluorescence detector (8 μl flow cell maintained at 45°C), and a Dionex Corona Veo charged aerosol detector (all from Thermo Fisher Scientific...