To create an enzyme, the cell must first transcribe the gene in the DNA into messenger RNA. The transcription is performed by an enzyme called RNA polymerase. RNA polymerase binds to the DNA strand at the promoter, unlinks the two strands of DNA and then makes a complementary copy of one...
For this reason, AMV RT is often used to reverse transcribe RNAs with strong secondary structure. Like other RTs, AMV RT is compatible with gene-specific primers, oligo(dT)15 primers or random hexamers, although use of random hexamers requires a reduced reaction temperature of 37°C. Gene-...
While an mRNA is created in the process of transcription, the directly transcribed product of a gene is not the final product that will exit the nucleus and undergo translation in order to create a protein. Such a "pre-mRNA" must first be modified....
A sequence in the DNA called the promoter determines which strand of DNA will be the used as the template for transcription of a particular gene. The... Learn more about this topic: mRNA Transcription Process & Phases from Chapter 9/ Lesson 2 ...
Whereas in the case of a parallel point mutation, a single base position has to repeat independently, in the case of recurrent gene fusion, multiple similar breakpoints must repeat independently. Mathematically, if the probability of the former is small, the probability of the latter is negligible...
The time taken to transcribe most metazoan genes is significant because of the substantial length of introns. Developmentally regulated gene networks, wher... IA Swinburne,PA Silver - 《Developmental Cell》 被引量: 136发表: 2008年 Quantitative Evaluation of the Exploration Strategies of a Mobile Rob...
The present invention is directed to novel replication-deficient adenoviral vectors characterized in that they harbor at least two lethal early region gene deletions (E1 and E4) that normally transcribe adenoviral early proteins. These novel recombinant vectors find particular use in human gene therapy...
The DMD gene is some 2100 kilobases long, and takes 16 hours to transcribe. Note that this is longer than it might take some developing neural cells to divide. What this means is that if cells are replicating faster than they can finish their protein expression, then there are bound to be...
Within the operon’s promoter is a short DNA sequence, called the operator, that is recognized by a transcription regulator. When this regulator binds to the ...
Dopamine is well recognized as a neurotransmitter in the brain, and regulates critical functions in a variety of peripheral systems. Growing research has a