More information on how to purify DNA Thermal cycler: Is the number of thermal cycles sufficient for your assay? Have you programmed the device correctly, and is it calibrated to ensure that it performs the reaction steps at the right temperatures? If the sequence of interest was amplified in...
Traditional vaccine purification schemes use multiple centrifugation and filtration steps to purify virus preparations. However, centrifugation poses certain limitations and is capital driven; other purification steps are time consuming and costly. Regulatory agencies have a stringent specification on the ...
In the grand scheme of your research, the resin you use to purify your construct seems like one of the more trivial decisions. After all, the resin your labmate uses seems to work great. However, every protein construct is a little different, so the resin that works for the rest of ...
We used antibody affinity capture to purify each viral sample directly on the grid without high speed centrifugation, using a procedure that avoids the disruption of surface glycoproteins associated with other purification methods25. We used our previous processing pipeline for subtomogram averaging of ...
The CLS Behring Fermentation Facility and Huck Institutes of the Life Sciences at the Pennsylvania State University provided equipment and training to grow and purify DHFR biological replicates. We thank T. Berek and P. Kashyap for help with growing and purifying the DHFR biological replicates....
The subcellular targeting of mRNAs encoding myelin proteins to the oligodendrocyte processes is an accepted fact in myelin formation. How these messengers are kept silent during their movement to the subcellular domain where they are turned on remains a mystery. This review focuses on aspects of mRNA...
Off to the LabTypically, when a healthcare provider collects your sample, they’ll send it off to a central lab where lab personnel will extract and purify RNA from the SARS-CoV-2 virus off your NP swab. This process can take hours for a professional biologist or lab technician to ...
Once the pooled samples are placed on 96-well plates – three of the wells are used for controls, and 93 are active pools – they’re subjected to a nucleic acid extraction, to purify viral RNA from the samples. The viral RNA is then tested using PCR to detect SARS-CoV-2. ...
We used male or female mice aged 6–8 weeks to purify primary T cells from the spleen. After the 2C transgenic mice were sacrificed via CO2 induction with a fill rate of 1.7–3.9 L/min with 30–70% of the chamber filled per minute, the spleen was harvested to isolate T cells....
Purification was not dissimilar to that achieved by affinity purification, although the protein was not purified to homogeneity33. Nevertheless orthogonal polishing steps may then be used to purify further. Also, for many industrial processes, such as nutritional supplementation or biotransformations, ...