The circularized streptavidin RNA aptamer retained its binding of streptavidin and was stabile in HeLa cell extracts compared to the linear form of the streptavidin aptamer. The self-spliced circular RNA from the transcribed permuted intron–exon transcripts in E. coli cells was purifi...
Thousands of eukaryotic protein-coding genes generate circular RNAs that have covalently linked ends and are resistant to degradation by exonucleases. To prove their circularity as well as biochemically enrich these transcripts, it has become standard in the field to use the 3′-5′ exonuclease RNas...
Messenger RNA (mRNA) is becoming an increasingly important therapeutic modality due to its potential for fast development and platform production. New emerging RNA modalities, such as circular RNA, drive the need for the development of non-affinity purification approaches. Recently, the...
TitleCircular RNA-GRIN2B Suppresses Neuropathic Pain by Targeting the NF-?B/SLICK Pathway CitationNeuroMolecular Medicine2024. AuthorsKun Wang, Zicong Shen, Xin Peng, Xiaotao Wu & Lu Mao TitleCircular RNA cVIM promotes hepatic stellate cell activation in liver fibrosis via miR-122-5p/miR-9-5p-...
Protocol Production and Purification of Artificial Circular RNA Sponges for Application in Molecular Biology and Medicine Janina Breuer and Oliver Rossbach * Institute of Biochemistry, Faculty of Biology and Chemistry, University of Giessen, Heinrich-Buff-Ring 17, D-35392 Giessen, Germany; janina....
Chromatin isolation by RNA purification (ChIRP) is a valuable method to map the genomic binding profile of chromatin-associated RNAs, such as long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs). ChIRP was initially designed by Chu and colleagues to investigate RNA bound DNA using affinity...
In this study, we address the isolation and genetic characterization of large circular plasmids from the bac- terial collection originated from a BPS. First, extrachromosomal DNA was isolated and purified, followed by high-throughput sequencing (Illumina sequencing technology, MiSeq system). The ...
The purified MBP-NU exhibited non-specific nuclease activity, able to degrade various nucleic acids, including RNA, single-stranded DNA and double-stranded DNA that was circular or linear. Its optimal temperature was 37 °C and optimal pH 8.0. From 1 L culture broth 10.8 mg NU could be ...
1. Creation of Lysate The first step in any nucleic acid purification reaction is releasing the DNA/RNA into solution. The goal of lysis is to rapidly and completely disrupt cells in a sample to release nucleic acid into the lysate. There are four general techniques for lysing materials: phys...
The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of subjecting an impure preparation comprising in vitro synthesized