Likewise, mitochondrial targeting signals (MTS) have been used to attach proteins to the mitochondria's outer membrane for many different reasons, e.g., to label mitochondria by use of fluorescent proteins (FPs) or sequestering proteins to the mitochondria membrane (see below). Experim...
in a label-free manner. As extensively described by Gosset et al.11, mechanical filters come in different shapes including weir-style filters, where a small planar gap is created allowing small cells to pass while retaining large cells. These type of filters have been used to filter white ...
Label-Free Detection of Single Protein Using a Nanoplasmonic-Photonic Hybrid Microcavity The signal-to-noise ratios in the experimental results associated with these proteins are 22:1 and 13:1, respectively. From the S/N in the BSA ... VR Dantham,S Holler,C Barbre,... - 《Nano Letters》...
This very recent label-free technique has been spurred by innovative advances in instrumentation and is driving new applications. MALDI-MSI has permitted to visualize and determine the regiospecific and temporal changes in proteins, peptides, lipids, glycolipids, drug molecules, and metabolites within ...
First recognized by Mitchell and co-workers40,41, it is now well established that this metabolic activation of APAP leads to extensive GSH depletion and then covalent binding of the reactive metabolite to proteins, which initiates the toxicity19. However, it is not the total protein binding in ...
Despite decades of accumulated knowledge about proteins and their post-translational modifications (PTMs), numerous questions remain regarding their molecular composition and biological function. One of the most fundamental queries is the extent to which the combinations of DNA-, RNA- and PTM-level var...
. Its molecular weight is 50 kilodalton. Pegaptanib adopts a threedimensional conformation that enables it to bind to extracellular VEGF. Pegaptanib binds to the major pathological VEGF isoform, extracellular VEGF165. It has no known immunogenicity unlike other anti–VEGF drugs which are proteins. ...
Novel lab-on-a-chip system for the label-free detection of DNA hybridization and protein-protein interaction by surface plasmon resonance (SPR) The detection of specific DNA sequences for the analysis of mutations as well as the detection of proteins gains increasing importance in the field of ...
Advanced prostate cancer remains one of the most common and deadly cancers, despite advances in treatment options. Immunotherapy has provided little benefit to a majority of patients, largely due to the immunosuppressive tumor microenvironment that gives
Since mAbs and proteins need protection from degradation in the systemic circulation, mAb-conjugated NPs or protein/mAb-loaded NPs can be excellent candidates for safe delivery of these therapeutics. Furthermore, the safety issues regarding the immunogenicity or toxicity of these agents can be ...