Left–right hemispheric asymmetry is an important aspect of healthy brain organization for many functions including language, and it can be altered in cognitive and psychiatric disorders. No mechanism has yet been identified for establishing the human brain’s left–right axis. We performed multivariate...
In Lesch–Nyhan disease (LND), deficiency of the purine salvage enzyme hypoxanthine guanine phosphoribosyl transferase (HGprt) leads to a characteristic neurobehavioral phenotype dominated by dystonia, cognitive deficits and incapacitating self-injurious behavior. It has been known for decades that LND is...
Biotin-labelled complementary RNA was obtained using the two-cycle eukaryotic target labelling assay and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays according to the standard protocol (Affymetrix, Santa Clara, CA, USA). GeneChip Operating Software (Affymetrix) was used for quality ...
Functional genetic variations in theCOMTgene modulate multiple spheres of mammalian behavior with similar findings from adult humans and mice.6,7,8,9,10,11,12However, if and how the effects ofCOMTgenetic polymorphisms might change during the course of development is still unclear, with mixed and...
Isotope-labelling experiments are coming of age. ISME J 2007;1(2):103-110. https://doi.org/10.1038/ismej.2007.30. Ginige MP, Hugenholtz P, Daims H, Wagner M, Keller J, Blackall LL: Use of stable-isotope probing, full-cycle rRNA analysis, and fluorescence in situ hybridization-micro...
2.4. [35S]-labelling of chlamydial proteins and 2D gel electrophoresis Infected HeLa cell cultures were incubated in a medium containing RPMI-1640, 10 μg/mL gentamicin, 40 μg/mL cycloheximide, 100 μCi/mL [35S]-methionine/cysteine (Promix, Amersham Pharmacia Biotech, Uppsala, Sweden) with ...
This cohort of patients without mutations in their collagen VI genes can be further divided into those which share abnormal collagen VI labelling in dermal fibroblasts cells and those in which collagen VI protein appears normal, emphasizing the need for careful assessment and diagnostic procedures for...
Biotin-labelled complementary RNA was obtained using the two-cycle eukaryotic target labelling assay and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays according to the standard protocol (Affymetrix, Santa Clara, CA, USA). GeneChip Operating Software (Affymetrix) was used for quality ...
Labelling of glycans: Labelling with 2-aminobenzamide acid (2-AB) was performed using the ludgerTag 2-AB glycan labelling kit. Briefly, DMSO was added to acetic acid in a 0.75:1 ratio and 100 μl added to 5 mg of 2-AB. This mixture is then added to the reductant sodium cyanobor...
To first assess both the potential for nonspecific expression and leakiness of the system, PostnMCM/ þ ; R26-eGFP mice were fed tamoxifen for 8 weeks, beginning at 8 weeks of age, but without injury (Fig. 1b). The data show o1% of interstitial cellular labelling in uninjured heart, ...