For most of the clones, ESTs have been generated at both ends and the corresponding sequence traces can be easily retrieved for quality checking[7]; furthermore, it is possible to obtain in a few days most of the EST cDNA clones, such as the ones generated by the IMAGE consortium and ...
sequence databases from the original authors, who volunteer their records to make the data publicly available or do so as part of the publication process. The NCBI GEO is intended to house different types of expression data, covering all type of sequencing data in both raw and processed formats...
After double enzyme cutting, this sequence was ligated to cucumber mosaic virus to construct VIGS recombinant vector, which was transformed to Agrobacterium tumefaciens. Finally, the Agrobacterium tumefaciens was used to infect the L. japonica seedlings. At the same time, the effects of Agrobacterium ...
On the sequence page you will find a wealth of information about that gene sequence, including the raw sequence towards the bottom of the page. What we want to do is to now open Primer-BLAST to design real-time PCR primers using this sequence. To do this, select the ‘Pick Primers’...
Current advanced deep-learning models have displayed an unprecedented level of comprehension and problem-solving ability, and have been used to predict gene structure, function and expression based on DNA or protein sequence, and prominently also their use in high-throughput phenomics in agriculture. ...
nearly all land plant sequences in clade A carry an N-terminal signal peptide and some of them have already been localized by mass spectrometry analyses to the cell wall [49]; both indicate ES-mediated targeting. This clade contains bothO. sativaαAmy3 and αAmy7, which have been shown ex...
2.2. Top 50 Most Abundant Transcripts in the Illumina 1st and 4th Leg Transcriptomes Table 1 and Table 2 list the top 50 most abundant transcripts identified in the Illumina 1st and 4th leg transcriptomes, respectively. All contigs had sequence similarity to genes in the Uniprot knowledgebase, ...
napus specific gene sequences from the Genbank (https://www.ncbi.nlm.nih.gov/genbank/ (accessed on 21 November 2021)) data base. Gene expression was measured by qPCR with the equipment described in 2.6. The reaction mixture contained the equivalent of 5 ng total RNA, 5 μL of 2× ...
在多年以前,那个基因组组装还没有白菜价,只有几个模式物种基因组的时代,对于一个未测序的基因组,研究者如果要研究某一个基因的功能,大多会通过同源物种相似基因设计PCR引物,然后去扩增cDNA. 如果是一个已知基因组的物种,如果要大规模识别基因, 研究者通常会使用EST(expressed sequence tags)序列。
The regions between the end of M4 and the beginning of the latch have the same ser-ala-pro (SAP) motif in all six species, even though the latch sequence in the invertebrates is quite different than that observed in vertebrates. This led to a search for sequence homology in this region ...