More than 1 200 000 entries generated from different tissues and species are now stored in the public EST database (dbEST)[4, 5], maintained at the National Center for Biotechnology Information (NCBI). Human and mouse sequences form the majority of the data held in this collection, with ...
Stein, L. Genome annotation: from sequence to biology. Nature Rev. Genet. 2, 493–503 (2001). Article CAS Google Scholar Pruitt, K.D. & Maglott, D.R. RefSeq and LocusLink: NCBI gene-centered resources. Nucleic Acids Res. 29, 137–140 (2001). Article CAS Google Scholar Burge, C...
It shows that although our dbBLAST runs faster than the file-based BLAST program for short query sequences, all implementations lack scalability. However, the results also indicate that stored procedures require significant less development effort—both in time and space—than traditional programming ...
The BLAST algorithm was developed as a way to perform DNA andprotein sequence similarity searches by an algorithm that isfaster than FASTA but considered to be equally as sensitive.Both of these methods follow a heuristic (tried-and-true) methodthat almost always works to find related sequences ...
New techniques, such as the multiplication of the required gene area in a test tube as well as RNase protection assays have been used successfully to pick out about 80-90% of the mutations which occur in one gene area and cause the disease of this gene area. The increased specificity and...
researchers have known that a gene calledFT O was related to obesity, but they didn't know how, and could not tie it to appetite(食欲)or other known causes.Now experiments show that a faulty version(缺陷型) of the gene causes energy from food to be stored as fat rather than burned. ...
insertions.27 These comprise 29 mutations of the XIAP gene reported in a Japanese study as well.41 For identification of gene mutations, "next-generation" whole- exome DNA sequencing or sequencing of specific candidate disease genes might be used to sequence XIAP and other IBD candidate ...
The whole premise of “evolution” hinges on impossibilities. The “engine of evolution” according to evotards is the “duplication” mutation that makes copies of all or parts of a genetic protein sequence. There are impossibilities associated with this ridiculous premise. Therefore it cannot and...
This guide introduces restriction enzymes, providing in-depth reference information and tools to help you find buffers for double digests, or find enzymes by name or recognition sequence. Restriction Enzyme Tools are available for desktop or mobile....
These small deletions result in a frame-shift that disrupts the ORFs within coding genes, thereby disrupting protein synthesis. LncRNAs do not contain ORFs and span tens of kilobases of sequence in size. As such, targeting them with a single gRNA is thought to be insufficient to disrupt their...