An mRNA has the sequence 5'-AUGAAAUCCUAG-3'. What is the template DNA strand for this sequence? a. 5'-TACTTTAGGATC-3' b. 5'-ATGAAATCCTAG-3' c. 5'-GATCCTAAAGTA-3' d. 5'-TACAAATCCTAG-3' e. 5'-CTAGGATTTCAT-3' What
The RNAs are reverse transcribed using labeled nucleotides and target specific, oligo dT, or random-sequence primers to create two labeled cDNA populations. The two cDNAs are hybridized to two identical arrays. After washing, the hybridized signal on each array is dete...
The main advantage of real-time PCR over PCR is that real-time PCR allows you to determine the initial number of copies of template DNA (the amplification target sequence) with accuracy and high sensitivity over a wide dynamic range. Real-time PCR results can either be qualitative (the presen...
Next-generation sequencing (NGS) is a technology for determining the sequence of DNA or RNA to study genetic variation associated with diseases or other biological phenomena. Introduced for commercial use in 2005, this method was initially ...
AMPINEXT™ DNA Library Preparation Kit (Illumina) ENZ-GEN504 Complete set of optimized reagents to carry out a successful DNA library preparation. Assay Time2 hours 30 minutes AMPINEXT™ DNA Size Selection Kit ENZ-GEN506 A complete set of optimized reagents for quick removal of DNA fragments...
The RNA CS (RCS) is the RNA Calibration Strand is the Enolase II from YHR174W. The transcript is 1,314 nucleotides long and also includes an additional 30-nucleotide poly(A) tail. The reference fasta file for the YHR174W ENO2 mRNA is available for download here. RCS is included in...
Fragmentation is followed by a reverse transcription reaction which converts the fragmented RNA into cDNA. Once this is completed, adapters and sample-specific indexes are added to the cDNA. The now indexed cDNA undergoes purification/clean-up, generating ready-to-sequence library molecules. Depending...
Prior to RNA-Seq, the main technology for RNA analysis and gene expression profiling was microarrays. The main limitation of this technology is that it can only detect predefined RNA sequences, providing a biased view of the transcriptome. RNA-Seq is not limited to known sequences and produces...
Behind this data is our Quanum®suite of technology solutions that enables secure and seamless ordering, processing, and sharing of test results to ensure that healthcare providers can access information easily and quickly make important care decisions. ...
9. Reverse transcriptase is used to convert RNA to cDNA. 10. Amplify in a strand-by-strand fashion. 11. Reads from a sequence. 12. determine the translational profile, align the sequence results to the genomic sequence. Applications of Ribosome Profiling ...