Swarup G: How to design a highly effective siRNA. J Biosci 2004, 29 (2) : 129-131. PubMed Abstract Publisher Full TextSwarup,G. (2004) How to design a highly effective siRNA. J. Biosci., 29, 129-131.Swarup, G. (2004) How to design a highly effective siRNA, J Biosci,...
How-To Perform siRNA Transfection How-To Optimize Gene Synthesis for Better Protein Expression How-To Create a Genetic Element or Construct How-To Extract Plasmid DNA How To Run the Bolt Tank and Gel How To use the DynaMag™-5 Magnet ...
To develop safe and powerful delivery vehicles suited for mRNA delivery, the mRNA field is currently strongly capitalizing on the vast knowledge gained during the clinical development of small interfering RNAs (siRNA). Efficient systemic delivery of siRNA can be achieved by its encapsulation into so ...
Cullis helped design these systems. In the 1980s, he was working on lipid nanoparticles (LNPs) as a delivery system for cancer drugs, but as interest in gene therapy began to take off in the 1990s he moved in that direction. He thought the tiny capsules would be an ideal way to protect...
CONTROLLING RNA Using small oligonucleotides, such as endogenous (miRNA) (Fabian et al., 2010), synthetic RNA (shRNA, siRNA), or other nucleic acid analogs (locked nucleic acids LNA, morpholino, 2′- O-methyl RNA oligo) (Cooper et al., 2009), it is possible to block gene ...
With the intensification of the greenhouse effect, a series of natural phenomena, such as global warming, are gradually recognized; when the ambient temperature increases to the extent that it causes heat stress in plants, agricultural production will in
“In my mind, one of the heroes in this story is the RNA nanoparticle,” said Daniel Anderson, professor of chemical engineering at the Massachusetts Institute of Technology, because “siRNA led to mRNA vaccines.” The FDA’s approval of the first RNAi-based drug was “proof these nanoparticl...
The pharmacological effect of the drug molecules can be compared to the effect of genetic alteration of the target using siRNA knockdown or CRISPR gene editing11,12. However, validation of drug target is still challenging since drug's pharmacological effect (efficacy and toxicity) may be resulted ...
10 years before [44], while the same approach aiming to target the AF4-MLL fusion transcript has failed because of the extremely long half-life of the AF4-MLL fusion protein complex (∼96 h) in contrast to the short-term effects of siRNA-mediated knockdowns (∼48 h) [45], [46]...
Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol. 2011;29:341–5. Article CAS PubMed Google Scholar Kojima R, Bojar D, Rizzi G, Hamri GC-E, El-Baba MD, Saxena P, et al. Designer exosomes produced by implanted cells intracerebrally deliver...