How to choose the best secondary antibody for your application.Secondary antibodies facilitate the indirect detection of target molecules by binding to the primary antibody, which in turn binds to the target antigen. Secondary antibodies are generally labeled with a tag to facilitate detection or ...
It is possible to use conjugated primary antibodies, but secondary antibodies provide many advantages. Using a secondary antibody makes it easy to choose a different conjugate no matter the primary antibody. For example, the same primary can be used with a secondary antibody con...
6. Cross-adsorbed antibodies– Cross-adsorbed secondary antibodies are traditional polyclonal secondaries that have undergone additional purification steps to eliminate cross-reactivity against unintended IgGs from non-target species. Cross-reactivity can lead to high background or non-specific signal when ...
Antibody-producing cells undergo a process of differentiation and class switch recombination (CSR) such that the antibodies produced start as immunoglobulin M (IgM) and then switch to IgG and IgA as the concentration of antigen changes and as the cells differentiate. Omori et al. show that CSR ...
We recommend that you use 5% milk in TBST, shaken for one hour at room temperature, to block all of our non-conjugate primary antibodies. This includes phospho-specific and total antibodies. Now I can already hear the clicking, comments, and hashtags, asking about "what about the phosphatases...
In contrast to other formats for monitoring phosphorylation, ICC is usually the method of choice for determining intracellular localization (View phospho-specific antibodies for ICC/IHC). Both flow cytometry and ICC/IHC require high-affinity and high-specificity antibodies, blocking steps, controls, ...
One option for funding your education is to preserve your main source of income. Many graduate students choose to earn a master’s degree online while they work. Otherwise, you may be able to secure financial aid by following these steps: ...
we review the management of uncommon clinical entities including nodular lymphocyte-predominant Hodgkin lymphoma, hairy cell leukemia, splenic lymphoma and primary lymphoma of extranodal sites. Finally, we include a perspective on novel targeted therapies, antibodies, antibody–drug conjugates, bispecific T...
Consider the cell frequency of your population of interest; this will indicate how many events you should collect to obtain meaningful data The antibodies Ensure your chosen antibodies have been validated for flow cytometry; always perform your own validation to optimize for your system ...
It is possible to use conjugated primary antibodies, but secondary antibodies provide many advantages. Using a secondary antibody makes it easy to choose a different conjugate no matter the primary antibody. For example, the same primary can be used with a secondary antibody con...