This product is a ready-to-use buffer, optimized for the dilution and incubation of both conjugated and unconjugated antibodies in flow cytometry assays (F). The buffer is formulated with 1X phosphate-buffered saline (PBS) compatible for use with live or fixed cells, and contains bovine serum...
R21912-50 通用Buffer Annexin Binding Buffer(5×),for flow Cytometry 50ml R21951-30 通用Buffer Antibody Dilution Buffer 30ml R21951-100 通用Buffer Antibody Dilution Buffer 100ml R21936-100 通用Buffer Antibody Penetration Buffer 100ml T1231-100 通用Buffer ABTS缓冲液(pH=5.0) 100ml T1231-500 通用...
All donor blood groups were subtyped by our blood bank using a lectin-based dilution assay. To detect recipient anti-A2 antibody titers we used a tube hemagglutination method. A/B antibody titer analysis by flow cytometry incubated serially diluted serum samples with donor erythrocytes. Each ...
Sino Biological Flow Cytometry antibodies are verified by WB, IF and IHC. For example, the human CD147 FCM MAb was verified by WB, IF and IHC.
A2B5 Antibody (433110) in FlowFlow cytometry analysis of A2B5 was performed using U-87 MG cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. ...
Antibody titration is also an important optimization technique for multiparameter flow cytometry and is the best way to minimize nonspecific binding and increase signal detection. It can also be used to minimize spillover spreading, which occurs...
Flow cytometry sample preparation As in any antibody-based technique, biological samples require preparation prior to staining and analysis. Cells require disassociation and are often fixed and permeabilized when analyzing cytoplasmic and nuclear proteins. Samples need to be washed after staining to remove...
Figure 5. Dilution of antibodies for sample staining in flow cytometry. 1X10^6 human peripheral blood lymphocytes were surface-stained with APC-Mouse IgG2b isotype control (1) or APC-Anti-Human CD4 (APC-65134, clone OKT4) using 2-fold serial dilutions (2-10). Samples were not fixed....
Selecting antibodies that are verified for flow cytometry is a key step toward experimental success. Antibody datasheets should show clear validation data, including details of the immunostaining protocol that was used, and should provide recommended antibody dilutions that can be used as a starting po...
Flow Cytometry Sample Human PBMC Primary Incubation 30 minutes at room temperature Blocking Agent PBS/BSA Secondary Incubation None Tertiary Incubation None Detection v-780/60 Results Summary This antibody hasn't worked very well at all, with really low specific signal and quite high...