Methods: In this study, several mouse strains reflecting a spectrum of primary antibody deficiency (IgA-/-, Aicda-/-, CD19-/- and JH-/-) were used to generate a functional small-bowel-specific cellular atlas using a novel high-parameter flow cytometry approach that allows ...
✓ Developed for flow cytometry (FACS) ✓ Tested with appropriate isotype control ✓ No need for secondary antibodies ✓ Conjugated to “extracellular” antibodies ✓ Cell-surface detection of proteins ✓ Permeabilization & cell fixation not required ✓ Time saving Within our antibody portfol...
Flow Cytometry is a modern multi-parameter cell analysis technology that can detect single particles at high speed, accurately and objectively. At present, in the early stage of drug development, especially for anti-tumor drugs, complex cell analysis through flow multicolor detection technology can pr...
Learn more: UltraComp compensation beads protocols for flow cytometry AbC Total Antibody Compensation kit AbC compensation bead kits contain two types of specially modified polystyrene microspheres: 1) AbC capture beads (also called positive ...
Beads were stained with 0.25 µg of each antibody and analyzed by flow cytometry. Each histogram represents one staining antibody. Learn more: UltraComp compensation beads protocols for flow cytometry AbC Total Antibody Compensation kit AbC compensation bead kits ...
Thus, it is recommended to follow protocols or suggestions by the antibody manufacturer. Before performing fixation and permeabilization steps, first prepare samples (from tissue homogenate or a cell line) to generate a single cell suspension, see our complete guide to flow cytometry. The fix, ...
Originally developed in the late 1960s, flow cytometry is a popular analytical cell-biology technique that utilizes light to count and profile cells in a heterogenous fluid mixture. Flow cytometry is a particularly powerful method because it allows a researcher to rapidly, accurately, and simply ...
Cell cycle progression can be studied with computational models that allow to describe and predict its perturbation by agents as ionizing radiation or drugs. Such models can then be integrated in tools for pre-clinical/clinical use, e.g. to optimize kine
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摘要: This presentation reports on the routine use of flow cytometric techniques in suspension cultures. As a model system an secreting hybridoma line was used to monitor (a specific ) and proliferative status as obtained by flow cytometry (FCM) and counting.关键词:...