If you know the concentration of enzyme sites you've added to the assay (Et) then you can calculate the catalytic constant Kcat. It is defined to equal Vmax/Et. Vmax and the Y values (enzyme velocities) are expressed in units of concentration per time, and Et must be entered in those ...
声明: 本网站大部分资源来源于用户创建编辑,上传,机构合作,自有兼职答题团队,如有侵犯了你的权益,请发送邮箱到feedback@deepthink.net.cn 本网站将在三个工作日内移除相关内容,刷刷题对内容所造成的任何后果不承担法律上的任何义务或责任
Technologies to improve weaning foods in developing countries. for the assay and kinetic characterization of exoglycosidases was demonstrated by performing parallel measurements of galactose using an established coupled-enzyme assay, and using these values to calculate Km and Vmax values against lactose.....