A method of estimating the elimination half-life of a drug at any selected serum concentration when the Km and Vmax of that drug are known is described. The method was validated in six patients by determining their Km and Vmax values for phenytoin, using data obtained at two serum ...
The method proposed here consists of a continuously flowing hydroponic system and a method of data analysis that estimates a value of Kmand Vmax for each plant. The method is nondestructive; it does not require large amounts of space; and the plants are near steadystate uptake. In simulated ...
Examining enzyme kinetics is critical for understanding cellular systems and for using enzymes in industry. The Michaelis-Menten equation has been widely used for over a century to estimate the enzyme kinetic parameters from reaction progress curves of substrates, which is known as the progress curve...
estimates of the symmetric and total low-level wind fields in tropical cyclones, constructed from estimations of an azimuthally averaged radius of maximum wind (RMAX), a symmetric tangential wind speed at a radius of 182 km (V182), a storm motion vector, and the maximum intensity (VMAX). Th...
Estimation of Vmax in auxotonic systoles from the rate of relative increase of isovolumic pressure: (dP-dt)kP. High speed oscilloscopic recordings (4000 mm/sec) of left ventricular pressure (micromanometer) and its first derivative were used to calculate contractile... MJ Wolk,JF Keefe,OHL ...
In 18 cats, Vmax for ethanol was 93 +/- 7 mumol.min-1 per 100 g liver or 21 +/- 2 mumol.min-1.kg body weight-1 and Km was 144 +/- 19 microM in terms of logarithmic mean sinusoidal concentration. At the dose of 8 mumol.min-1.kg body weight-1 used for estimation of ...
When these data were analyzed using conventional linear regression of double‐reciprocal plots, the range of Km and Vmax values obtained by different students varied widely. Even worse, some students obtained negative values for the kinetic parameters. Although such a scenario could make us think of...
To our knowledge, this is the first effort to determine both Vmax and Km kinetic parameters describing the biotransformation of phenanthrene- and anthracene-analogue azaarenes. The results show that the rate of removal of azaarenes from contaminated soil will probably be rather high, although ...
For 9-methyl-acridine only, a k1 of 0.6 L/g total protein/h was estimated and for benzo[h]quinoline, the highest biotransformation rate measured was 6.8 +/- 0.4 mg/g total protein/h. To our knowledge, this is the first effort to determine both Vmax and Km kinetic parameters describing...
The Kmand Vmax values for total bilirubin glucuronide formations were determined to be 0.05 ± 0.01 μM and 181.9 ± 5.3 pmol/min/mg-protein, respectively, in human recombinant UGT1A1, and 0.23 ± 0.05 μM and 875 ± 45 pmol/min/mg protein in human liver microsomes (HLM).Conclusion: ...