For personal use only in study and research; not for commercial use 酶动力参数Km、Vmax和Kcat值的计算(以日立U3010为例) 强玮2014-3-31 一、Km 根据酶促反应方程,即米-曼式氏方程(Michaelis-Menten equation): V =(VmaxS)/(Km + S) 当v=0.5Vmax时,Km=[S],可见Km等于酶促反应的初速率为最大...
Michaelis-Menten type equationmaximum reaction rateMichaelis constantinitial steady-state reaction rateend point method/ A8220P Measurements of chemical rate constants, reaction cross sections, and activation energies A8220W Computational modelling of chemical kinetics C7320 Physics and chemistry computing C...
have a hydrogen content of 8.0 percent (by volume) or greater, and are designed for and operated with an exit velocity less than 37.2 m/sec (122 ft/sec) and less than the velocityVmax, as determined by the following equation:Vmax=(XH2-K1)* K2 Where:Vmax=Maximum permitted velocity, m...
1.Aim To explore the effects of solanine on Km and Vmax of NATase of HepG2.目的探讨龙葵碱对HepG2细胞NAT酶米氏常数Km及最大反应速率Vmax的影响。 2.Linewaver-Burk Equation is mostly used to draw a figure of 1/v～1/[S] to work out Km and Vmax presently.当前仍多用作图法求解酶动力学...
米氏方程(Michaelis-Menten equation)是表示一个酶促反应的起始速度与底物浓度关系的速度方程。[1] 底物浓度 [S] 对酶反映速度的影响 在酶促反应中,当底物浓度非常大时,反应速度接近于一个恒定值。在曲线的这个区域,酶几乎被底物饱和,反应相对于底...
One transformation of the Michaelis-Menten equation is the Lineweaver-Burk, or double-reciprocal, equation. Multiplying both sides of the Lineweaver-Burk equation by Vmax and rearranging gives the Eadie-Hofstee equation. С Slope =-KM Vo: V - (Km) ...
The term Km in enzyme activity is discussed under Michaelis-Menten kinetics. It is a common model of enzyme kinetics. This model was named after German biochemist Leonor Michaelis and the Canadian physician Maud Menten. This model is expressed as an equation. ...
For personal use only in study and research; not for commercial use 酶动力参数Km、Vmax和Kcat值的计算(以日立U3010为例) 强玮2014-3-31 一、Km 根据酶促反应方程,即米-曼式氏方程(Michaelis-Menten equation): V =(VmaxS)/(Km + S) 当v=0.5Vmax时,Km=[S],可见Km等于酶促反应的初速率为最大速...
，因此可以把平衡态看成是稳态的一个特例。为了纪念Michaelis和Menten两人，人们把上述带三角符号的的方程都称为米-曼氏方程（Michaelis-Menten equation）。参数意义 ①当 时， 。因此，Km等于酶促反应速度达最大值一半时的底物浓度。②当 时， =Ks。因此，Km可以反映酶与底物亲和力的大小，即 值越小，则酶...