For personal use only in study and research; not for commercial use 酶动力参数Km、Vmax和Kcat值的计算(以日立U3010为例) 强玮2014-3-31 一、Km 根据酶促反应方程,即米-曼式氏方程(Michaelis-Menten equation): V =(VmaxS)/(Km + S) 当v=0.5Vmax时,Km=[S],可见Km等于酶促反应的初速率为最大...
药物代谢的米氏常数Km和酶系统的最大速率Vmax值对于了解药物(特别是具有剂量依赖药动学性质的药物)的临床药动学性质和剂量范围至关重要.本文讨论的方法是用Michaelis-Menten方程的变式和在两个完全不同的血药浓度时示踪研究所得数据来测定体内药物的Km和Vmax值.作者用已知具有剂量依赖药动学性质的苯妥英(PHT)进行人...
Michaelis-Menten type equationmaximum reaction rateMichaelis constantinitial steady-state reaction rateend point method/ A8220P Measurements of chemical rate constants, reaction cross sections, and activation energies A8220W Computational modelling of chemical kinetics C7320 Physics and chemistry computing C...
1.Aim To explore the effects of solanine on Km and Vmax of NATase of HepG2.目的探讨龙葵碱对HepG2细胞NAT酶米氏常数Km及最大反应速率Vmax的影响。 2.Linewaver-Burk Equation is mostly used to draw a figure of 1/v～1/[S] to work out Km and Vmax presently.当前仍多用作图法求解酶动力学...
The Henderson-Hasselbalch equation can be used for: a. Estimating the Vmax and Km b. Creating a standard curve c. Estimating pH values of a buffer solution when the concentration of the conjugate acid and conjugate base are known d. Calculating the ab ...
have a hydrogen content of 8.0 percent (by volume) or greater, and are designed for and operated with an exit velocity less than 37.2 m/sec (122 ft/sec) and less than the velocity Vmax, as determined by the following equation: Vmax=(XH2−K1)* K2 Where: Vmax=Maximum permitted ...
Enzyme Kinetics Equation | Definition & Examples from Chapter 6 / Lesson 5 51K Learn how to measure enzyme activity. Discover the factors that affect enzyme kinetics, and work through examples of the Michaelis-Menten enzyme equation. Related...
One transformation of the Michaelis-Menten equation is the Lineweaver-Burk, or double-reciprocal, equation. Multiplying both sides of the Lineweaver-Burk equation by Vmax and rearranging gives the Eadie-Hofstee equation. С Slope =-KM Vo: V - (Km) ...
，因此可以把平衡态看成是稳态的一个特例。为了纪念Michaelis和Menten两人，人们把上述带三角符号的的方程都称为米-曼氏方程（Michaelis-Menten equation）。参数意义 ①当 时， 。因此，Km等于酶促反应速度达最大值一半时的底物浓度。②当 时， =Ks。因此，Km可以反映酶与底物亲和力的大小，即 值越小，则酶...