These rate differences are such that the addition of a small excess of enzyme will avoid any problems due to incomplete digestion. As always, however, one must be aware of the experimental molar concentration of recognition sites and digest conditions relative to that of the unit definition. See...
Owing to this very strong interaction and the high protein concentrations required in the NMR experiments, we employed fluorescence spectroscopy to avoid ligand depletion effects and to ultimately determine the dissociation constant, Kd, of albicidin binding to AlbAS and AlbAL. The experimental read-...
Processing of genome 5’ termini as a strategy of negative-strand RNA viruses to avoid RIG-I-dependent interferon induction PLoS One, 3 (2008), p. e2032 CrossrefView in ScopusGoogle Scholar [28] H. Kato, et al. Differential roles of MDA5 and RIG-I helicases in the recognition of RNA...
software also BLASTs the primer sequence against the genome of the organism of interest. This means that the software will tell you if the primers are likely to bind elsewhere in the genome, which you obviously want to avoid. Make sure your primer pairs are specific for your intended target...
For example, Mason-Pfizer Monkey Virus (MPMV) uses a structured RNA element termed constitutive transport element (CTE) that employs the cellular NXF1/Tap export pathway to avoid nuclear retention of intron-containing RNAs (Bray et al., 1994). This pathway is Crm1-independent, as treatment ...
Regardless, the Panasonic unit is difficult to find, as are filters, so purified or distilled water can be used in substitution. Water molecules can move across the cell membrane, not just via aquaporins, albeit at a slower speed. To avoid hyponatremia, or water intoxication, in both cases,...
Next, 6-mercapto-1-hexanol is added to cover the empty spaces and avoid unspecific DNA binding. Capture DNA is hybridized with complementary strand labelled with rhodamine B on the 5′ end. This structure permitted the detection of BRCA1 breast cancer gene. A very similar approach, but with ...
FTSJ3 is an RNA 2′-O-methyltransferase recruited by HIV to avoid innate immune sensing. Nature 2019, 565, 500–504. [Google Scholar] [CrossRef] [PubMed] Sharma, A.; Yilmaz, A.; Marsh, K.; Cochrane, A.; Boris-Lawrie, K. Thriving under Stress: Selective Translation of HIV-1 ...
These rate differences are such that the addition of a small excess of enzyme will avoid any problems due to incomplete digestion. As always, however, one must be aware of the experimental molar concentration of recognition sites and digest conditions relative to that of the unit definition. See...